Department of Pharmaceutical Biochemistry

Główne kierunki badawcze 

1. Fulereny jako nanonośniki leków przeciwnowotworowych - badania in vitro
2. Badania nad metalotioneiną: rola antyoksydacyjna i detoksykacyjna, oddziaływanie z innymi białkami transportującymi metale istotne dla homeostazy organizmu, ekspresja izoform metalotioneiny w chorobach trzustki.
3. Znaczenie wybranych polimorfizmów genów syntaz tlenku azotu (NOS) oraz genu kodującego enzym konwertujący angiotensynę (ACE) w ocenie ryzyka wystąpienia cukrzycowej choroby nerek.
4. Wpływ dymu tytoniowego na zaburzenie funkcji trzustki u osób zdrowych i pacjentów z chorobami tego narządu.
5. Status pro/antyoksydacyjny we krwi ludzi narażonych na ksenobiotyki środowiskowe.
6. Ocena skutków palenia papierosów na wydolność fizyczną zdrowych osób
7. Izolacja i charakterystyka receptora hemoglobiny z hepatocytów.
8. Glikacja cystatyny białka jaja kurzego (owocystatyny) i badanie jej właściwości,
   w tym zmian konformacyjnych i hamowania leguminazy.
9. Wpływ antybiotyków aminoglikozydowych na wiązanie kurzej cystatyny
    w kanalikach nerkowych.
10. Badanie procesów glikacji i stresu oksydacyjnego w cukrzycy typu 2.
11. Ocena właściwości antyglikooksydacyjnych leków w badaniach in vitro.
12. Poszukiwanie testów biochemicznych użytecznych w diagnostyce i monitorowaniu terapii przewlekłych chorób wątroby.

Lata 2021-2022

1. Association Of Genetic Variants In IL6 Gene (Rs1800795) With The Concentration Of Inflammatory Markers (IL-6, Hs-CRP) And Superoxide Dismutase In The Blood Of Patients With Acute Pancreatitis - Preliminary Findings, Monika Ołdakowska, Milena Ściskalska, Marta Kepinska, Grzegorz Marek, Halina Milnerowicz. Genes 2022 Vol.13 No.2 Art.290

2. Association Of Genetic Variants In The GPX1 And GPX4 Genes With The Activities Of Glutathione-Dependent Enzymes, Their Interaction With Smoking And The Risk Of Acute Pancreatitis. Milena Ściskalska, Halina Milnerowicz. Biomed.Pharmacother. 2022 Vol.146 Art.112591

3. Effect Of Biosynthesized Silver Nanoparticles On Bacterial Biofilm Changes In S. Aureus And E. Coli. Bozena Hosnedlova, Daniil Kabanov, Marta Kepinska, Vedha Hari B Narayanan, Arli Aditya Parikesit, Carlos Fernandez, Geir Bjorklund, Hoai Viet Nguyen, Awais Farid, Jiri Sochor, Agnes Pholosi, Mojmir Baron, Milan Jakubek, René Kizek. Nanomaterials 2022 Vol.12 No.13 Art.2183

4. LAG-3 As A Potent Target For Novel Anticancer Therapies Of A Wide Range Of Tumors. Natalia Sauer, Wojciech Szlasa, Laura Jonderko, Małgorzata Oślizło, Dominika Kunachowicz, Julita Kulbacka, Katarzyna Karłowicz-Bodalska. Int.J.Mol.Sci. 2022 Vol.23 No.17 Art.9958

5. Structural Changes In Selected Human Proteins Induced By Exposure To Quantum Dots, Their Biological Relevance And Possible Biomedical Applications. Dominika Kunachowicz, Milena Ściskalska, Milan Jakubek, René Kizek, Marta Kepinska. Nanoimpact 2022 Vol.26 Art.100405

6. The Application Of Nanoparticles In Diagnosis And Treatment Of Kidney Diseases. Patrycja Paluszkiewicz, Adrian Martuszewski, Natalia Zaręba, Kamila Wala, Mirosław Banasik, Marta Kepinska. Int.J.Mol.Sci. 2022 Vol.23 No.1 Art.131

7. The Changes In Stress Coping, Alcohol Use, Cigarette Smoking And Physical Activity During COVID-19 Related Lockdown In Medical Students In Poland. Aureliusz Kosendiak, Magdalena Król, Milena Ściskalska, Marta Kepinska. Int.J.Environ.Res.Public Health 2022 Vol.19 No.1 Art.302         

8. The Impact Of Fullerenes As Doxorubicin Nano-Transporters On Metallothionein And Superoxide Dismutase Status In MCF-10A Cells. Natalia Zaręba, Klaudia Więcławik, René Kizek, Bozena Hosnedlovamarta Kepinska. Pharmaceutics 2022 Vol.14 No.1 Art.102

9.  Activity of CdTe quantum-dot-tagged superoxide dismutase and its analysis in capillary electrophoresis. NATALIA ZARĘBA, ŁUKASZ LEWANDOWSKI, DOMINIKA KUNACHOWICZ, RENÉ KIZEK, MARTA KEPINSKA. Int.J.Mol.Sci. 2021 Vol.22 no.11 art.6156

10.  Activity of glutathione S-transferase and its π isoenzyme in the context of single nucleotide polymorphism in the GSTP1 gene (rs1695) and tobacco smoke exposure in the patients with acute pancreatitis and healthy subjects. MILENA ŚCISKALSKA, HALINA MILNEROWICZ. Biomed.Pharmacother. 2021 Vol.140 art.111589

11.  Concentration/activity of superoxide dismutase isozymes and the pro-/antioxidative status, in context of type 2 diabetes and selected single nucleotide polymorphisms (genes: INS, SOD1, SOD2, SOD3) - preliminary findings. ŁUKASZ LEWANDOWSKI, IWONA URBANOWICZ, MARTA KEPINSKA, HALINA MILNEROWICZ. Biomed.Pharmacother. 2021 Vol.137 art.       

12. Donor-derived cell-free DNA in kidney transplantation as a potential rejection biomarker: a systematic literature review. Adrian Martuszewski, Patrycja Paluszkiewicz, Magdalena Król, Mirosław Banasik, Marta Kepinska. J.Clin.Med. 2021 Vol.10 No.2 Art.193

13. Human nitric oxide synthase - its functions, polymorphisms, and inhibitors in the context of inflammation, diabetes and cardiovascular diseases. Magdalena Król, Marta Kepinska. Int.J.Mol.Sci. 2021 Vol.22 No.1 Art.56

14.  Importance of genetic polymorphisms in MT1 and MT2 genes in metals homeostasis and their relationship with the risk of acute pancreatitis occurrence in smokers - preliminary findings. Milena Ściskalska, Monika Ołdakowska, Halina Milnerowicz. Int.J.Mol.Sci. 2021 Vol.22 no.11 art.5725

15. The impact of early pregnancy and exposure to tobacco smoke on blood antioxidant status and copper, zinc, cadmium concentration - a pilot study. Anna Bizoń, Halina Milnerowicz, Katarzyna Kowalska-Piastun, Ewa Milnerowicz-Nabzdyk. Antioxidants 2021 Vol.10 No.3 Art.493

16. The summarized assessment of endothelin A receptors expression in renal transplant compartments associated with antibody-mediated rejection. Mirosław Banasik, Magdalena Kuriata-Kordek, Piotr Donizy, Katarzyna Nowańska, Krzysztof Wiśnicki, Krzysztof Letachowicz, Sławomir Zmonarski, Dorota Kamińska, Oktawia Mazanowska, Tomasz Dawiskiba, Dariusz Janczak, Agnieszka Hałoń, Marta Kepinska, Bartosz Uchmanowicz, Justyna Zachciał, Andrzej Tukiendorf, Magdalena Krajewska. Diagnostics 2021 Vol.11 No.12 Art.2366

17.  Theranostic approach for the protein corona of polysaccharide nanoparticles. Sylvie Skalickova, Pavel Horky, Veronika Mlejnkova, Jiri Skladanka, Bozena Hosnedlova, Branislav Ruttkay-Nedecky, Carlos Fernandez, René Kizek. Chem.Rec. 2021 Vol.2

Pharmacy year II (winter semester 2022/2023) - Biochemistry

Classes (stationary) - classroom of the Department of Pharmaceutical Biochemistry:
Monday ( 12⁴⁵ –15⁴⁵):     Gr. CL 1-4
Tuesday ( 8⁰⁰ – 11⁰⁰):              Gr. CL 5-8
Tuesday (12⁰⁰ – 15⁰⁰):             Gr. CL 9-12
Friday (8⁰⁰ – 11⁰⁰):                  Gr. CL 13-14

Lecture (online)
Thursday 30 hrs. (2 hrs. x 15 weeks) – 16³⁰-18⁰⁰ on-line (6.10.22-26.01.23)
Friday 10 hrs. (2 hrs. x 5 weeks) – 16³⁰-18⁰⁰ on-line (18.11.22-16.12.22)

Obligatory script: "Laboratorium z biochemii dla studentów farmacji" (Biochemistry laboratory for pharmacy students), ed. Jakub Gburek.

Disposable gloves, aprons, changeable footwear and a notebook should be brought to the classes.

Medical Analytics II (winter semester 2022/2023) - Biochemistry

Classes (stationary) - classroom of the Department of Pharmaceutical Biochemistry:
Wednesday (9⁴⁵ – 12⁰⁰):                  Gr. CL 1-4

Seminar (stationary)
środa (8⁰⁰ – 9³⁰)  (A2/8.2/005 summer semester Department of Pharmaceutical Biochemistry)
 

lecture (online)
Tuesday  30 hrs.  (2 hrs. x 15 weeks) – 16¹⁵-17⁴⁵ – on-line (11.10.22-24.01.23)

Obligatory script: "Biochemia laboratoryjna. Podręcznik dla studentów analityki medycznej" (Laboratory Biochemistry. Textbook for medical analytics students), ed. Agnieszka Piwowar.

Disposable gloves, aprons, changeable footwear and a notebook should be brought to the classes.

Medical Analytics year IV (winter semester 2022/2023) - Clinical Biochemistry

Classes (stationary) - classroom of the Department of Pharmaceutical Biochemistry:
Thursday  (8⁰⁰ – 11⁰⁰):            Gr. CL 1- 4

Lecture (online)
Thursday 17⁰⁰-18³⁰ , on line (06.10.22 – 08.12.22)
Thursday 15⁰⁰-16³⁰ , on line (15.12.22)
Thursday 15⁰⁰-16³⁰ , on line (22.12.22)
Thursday 12³⁰-14⁰⁰ , on line (12.01.23)
Thursday12³⁰-14⁰⁰ , on line (19.01.23)
Thursday 12³⁰-14⁰⁰ , on line (26.01.23)

Obligatory script: "Biochemia kliniczna : podręcznik do ćwiczeń dla studentów Oddziału Analityki Medycznej" (Clinical biochemistry: textbook for medical analytics students), ed. Jolanta Zuwała-Jagiełło.

Disposable gloves, aprons, changeable footwear and a notebook should be brought to the classes.

• Scientific Association at the Division of Biomedical and Environmental Analyses

  Facebook group link:https://www.facebook.com/groups/skn.toksykologia.umed.wroc

  Head: Marta Kepinska,
   PhD, professor at Wroclaw Medical University
   Natalia Zaręba, MSc

  Chair:
  Piotr Jędrzejewski
  piotr.jedrzejewski@student.umw.edu.pl

  Deputy Chair:
  Julia Kurzynoga
  julia.kurzynoga@student.umw.edu.pl

  Office assistant
  Marta Ligocka
  marta.ligocka@student.umw.edu.pl

  Characteristics of the scientific association:
  The purpose of the activities of the association is to expand the knowledge in the field of nanomedicine - especially based on silver nanoparticles, fullerenes and quantum dots, broadly defined toxicology including nanotoxicology, molecular biology and the impact of environmental threats on the human body. Thanks to the involvement of the members of the association in scientific research and their participation in scientific conferences, practical skills are combined with theoretical knowledge of students of all faculties of the University.

  Research areas:
  - Fullerenes as nanocarriers for anticancer drugs - in vitro studies;
  - Isolation of exosomes from human blood serum and analysis of their protein composition;
  - The role of polymorphisms of selected genes in the assessment of the risk of diabetic kidney disease;
  - Coupling quantum dots with proteins and analysis of their cytotoxicity – in vitro studies;
  - Damage to the vascular endothelium and the severity of inflammation in patients with acute pancreatitis - in vivo and in vitro studies.

  Recent research projects:
  FAST (EDITION II)
  Diabetes as a "lifestyle disease" - the importance of the nitric oxide synthase 3 polymorphism - rs799983 in type 2 diabetes risk assessment - the purpose of the project is the qualitative and quantitative testing of the nitric oxide synthase (NOS3) isoenzyme and checking whether selected changes in the genes encoding the nitric oxide synthase isoform affect parameters such as glucose and insulin levels.
  The project consists of analysing the occurrence of polymorphism in the NOS3 gene in people diagnosed with type 2 diabetes and/or obesity by measuring NOS3 levels and assessing one of the polymorphisms of the NOS3 gene - rs799983, which is associated with type 2 diabetes and obesity.

  FAST (EDITION IV)
  Synthesis and cytotoxicity of fullerene complexes with doxorubicin as a modern form of drug for targeted therapy of breast cancer - the project involves the assessment of the cytotoxicity of fullerenes (C₆₀) and their complexes with doxorubicin (DOX) on doxorubicin-resistant MCF7 breast cancer cells compared to healthy MCF10a mammary gland cells. The association members independently synthesise fullerene complexes with doxorubicin, measure their size and zeta potential, which corresponds to the surface charge of nanoparticles, conduct cell cultures and a cytotoxicity test showing the effect of synthesised complexes with therapeutic potential on in vitro models.
  The purpose of the project is to demonstrate the impact of fullerene complexes with doxorubicin on doxorubicin-resistant MCF7 breast cancer cells and the lack of toxicity against healthy MCF10a breast cells.