Katedra i Zakład Biologii i Parazytologii Lekarskiej

Department of Medical Biology and Parasitology

Address: ul. J. Mikulicza-Radeckiego 9, 50-345 Wrocław
e-mail: malgorzata.pekalska-cisek@umw.edu.pl
tel. 71 784 15 12 (office)
fax: 71 784 01 07

Head of the Department

Prof. Andrzej Hendrich
e-mail: andrzej.hendrich@umw.edu.pl
tel. 71 784 15 11

SCIENTIFIC RESEARCH

The scientific research carried out in recent years in the Department of Medical Biology and Parasitology covers two main thematic groups.

1. Microbiological research
   – use of plant-based preparations containing anthocyanins and other heterocyclic compounds against uropathogenic Escherichia coli, Pseudomonas aeruginosa and Enterobacter cloacae. The aim of the research is to look for agents that can reduce the possibility of urinary tract infections by bacteria. Research on the effect (or biological activity) of these preparations on the virulence characteristics and biofilm-forming ability of E. coli strains is being conducted at both phenotypic and genotypic levels using molecular biology methods.
   – use of photodynamic therapy to inactivate pathogenic bacteria for humans.

2. Parasitological research
   – occurrence of opportunistic parasites and immunological analysis in immunosuppressed patients. The research focuses on people living with HIV, patients with pharmacologically induced immunosuppression (organ recipients, children with cancer), as well as pregnant women and premature infants in the Lower Silesian population. Our research mainly focuses on the prevalence and molecular characteristics of microsporidia, Cryptosporidium spp. and Pneumocystis jirovecii. There is also research on the prevalence of free-living free-living amoebas, including those of the genus Acanthamoeba, in humans.
   – a separate group consists of research on the search for natural reservoirs of opportunistic parasites for humans in the zoonotic environment. The research is conducted using classical diagnostic techniques and molecular biology techniques.

LABORATORIES:

Parasitology Laboratory – head: Maria Wesołowska, PhD

The Laboratory performs research to confirm or exclude the presence of parasites (with particular emphasis on opportunistic ones) in patients with immunosuppression. For this purpose, standard and novel methods of immunoenzymatic and molecular diagnostics are applied. In addition, research is being conducted on tropical parasites, e.g. of the genus Plasmodium, which causes malaria, microfilariasis and leishmaniasis.

Molecular Biology Laboratory – head: Marta Kicia, PhD, Prof. of the Wroclaw Medical University

The Laboratory conducts molecular research in two thematic areas. On the one hand, the subject of research includes the identification and molecular characterization of parasites in immunocompetent and immunosuppressed patients. Our interests include both opportunistic and tropical parasites. In addition, the Laboratory conducts research on the molecular characteristics of Escherichia coli strains isolated from the urine of patients diagnosed with various clinical forms of urinary tract infections. In the implementation of research, we use methods based on both classical PCR and real-time quantitative PCR (qRT-PCR).

International

COOPERATION

• Respiratory Clinical Research Group and Pneumocystis Research Laboratory, University of Chile School of Medicine, Santiago, Chile (since 2018);

• Group of Opportunistic Protozoa/HIV and Other Protozoa, Unit of Medical Parasitology, Centre for Malaria and Other Tropical Diseases (CMDT), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Lisbon, Portugal (since 2014);

• Laboratory of Veterinary and Medical Protistology, Institute of Parasitology, Biology Centre, Academy of Science of the Czech Republic, Czeske Budejowice, Czech Republic (since 2012).

National

• Department of Internal Medicine with Horse, Dog and Cat Clinic, Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland;

• Department of Parasitology, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland;

• Units of the Wroclaw Medical University (Clinical Department of Nephrology and Transplantation Medicine, 2nd Clinical Department of Paediatrics, Gastroenterology and Nutrition, Clinical Department of Paediatric Bone Marrow Transplantation, Oncology and Haematology; Clinical Department of Pulmonology and Lung Oncology; Clinical Department of Physiotherapy and Ward of Trauma and Orthopaedic Surgery of the Provincial Specialist Hospital in Wrocław; Clinical Department of Neonatology; 2nd Department of Gynaecology and Obstetrics).

PUBLICATIONS

List of publications for 2023

List of publications for 2022

List of publications for 2021

List of publications for 2020

List of publications for 2019

List of publications for 2018

List of publications for 2017

List of publications for 2016

List of publications for 2015

List of publications for 2014

List of publications for 2013

SCIENTIFIC RESEARCH

Research conducted in the Department of Biology and Medical Parasitology in the recent years cover two main topics.

1. Microbiological research

– application of specimens of plant origin containing anthocyanins and other heterocyclic chemical compounds against uropathogenic Escherichia coli, Pseudomonas aeruginosa and Enterobacter cloacae. The aim of the research is to search for agents that may reduce the possibility of the urinary tract bacterial infection. Studies of their biological activity or impact on the virulence characteristics and the ability to produce biofilm by E. coli strains are conducted both at the phenotypic and genotypic level using molecular biology methods.

– the use of photodynamic therapy to inactivate bacteria pathogenic to humans.

2. Parasitological research

– the occurrence of opportunistic parasites and immunological analysis in immunosuppressed patients. The research mainly covers people living with HIV, people with pharmacologically induced immunosuppression (organ transplant recipients, children with cancer), as well as pregnant women and preterm newborns in the population of Lower Silesia. Our studies focus mainly on the prevalence and molecular characteristics of microsporidia, Cryptosporidium spp and Pneumocystis jirovecii. We also perform studies on the frequency of free-living amoebae, including Acanthamoeba, in humans.

–search for natural reservoirs of opportunistic parasites for humans in the zoonotic environment. The research is conducted using classic diagnostic techniques and molecular biology methods.
 

LABORATORIES

Laboratory of Parasitology – supervisor:  Maria Wesołowska, PhD

The laboratory performs research to confirm or exclude the presence of parasites (with particular emphasis on opportunistic ones) in patients with immunosuppression. For this purpose, standard and novel methods of immunoenzymatic and molecular diagnostics are applied. In addition, research is being conducted on tropical parasites, e.g. of the genus Plasmodium, which causes malaria, microfilariasis and leishmaniasis.

Laboratory of Molecular Biology – supervisor:  Marta Kicia, PhD

The laboratory conducts molecular research in two topics. On the one hand, the subject of research includes the identification and molecular characterization of parasites in immunocompetent and immunosuppressed patients. Our interests include both opportunistic and tropical parasites. In addition, the laboratory conducts research on the molecular characteristics of Escherichia coli strains isolated from the urine of patients diagnosed with various clinical forms of urinary tract infections. In the implementation of research, we use methods based on both classical PCR and real-time quantitative PCR (qRT-PCR).

COOPERATION

International

• Respiratory Clinical Research Group and Pneumocystis Research Laboratory, University of Chile School of Medicine, Santiago, Chile (since 2018);
   
• Group of Opportunistic Protozoa/HIV and Other Protozoa, Unit of Medical Parasitology, Centre for Malaria and Other Tropical Diseases (CMDT), Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Lisbon, Portugal (since 2014);
   
• Laboratory of Veterinary and Medical Protistology, Institute of Parasitology, Biology Centre, Academy of Science of the Czech Republic, Czeske Budejowice, Czech Republic (since 2012).

National

• Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland;
• Department of Parasitology, Institute of Genetics and Microbiology, University of Wroclaw, Wroclaw, Poland;
• Units of the Wroclaw Medical University (Department and Clinic of Nephrology and Transplantation Medicine; 2nd Department and Clinic of Paediatrics, Gastroenterology and Nutrition; Department of Paediatric Bone Marrow Transplantation, Oncology and Hematology; Department and Clinic of Pulmonology and Lung Cancers; Department of Physiotherapy and Nonresident Department of Traumatic-Orthopedic Surgery, Provincial Specialist Hospital in Wroclaw; Department and Clinic of Neonatology; 2nd Department of Gynaecology and Obstetrics).

PUBLICATIONS

List of publications for 2022

1. Korzekwa, K.; Kędziora, A.; Stańczykiewicz, B.; Bugla-Płoskońska, G.; Wojnicz, D. Benefits of usage of immobilized silver nanoparticles as Pseudomonas aeruginosa antibiofilm factors. Int. J. Mol. Sci. 2022, 23, 284.
2. Sycz, Z; Tichaczek-Goska, D.; Wojnicz, D. Anti-planktonic and anti-biofilm properties of pentacyclic triterpenes—asiatic acid andursolic acid as promising antibacterial future pharmaceuticals. Biomolecules 2022, 12, 98.
3. Kicia M., Zajączkowska Ż., Kvac M., Cebulski K., Holubova N., Wencel P., Mayer L., Wesołowska M., Sak B. Encephalitozoon cuniculi and extraintestinal microsporidiosis in bird owners. Emerg.Infect.Dis. 2022, 28(3), 705-708.
4. Szydłowicz M., Królak-Olejnik B., Vargas S.L., Zajączkowska Ż., Paluszyńska D., Szczygieł A., Matos O., Hendrich A.B., Kicia M. Pneumocystis jirovecii colonization in preterm newborns with respiratory distress syndrome. J.Infect.Dis. 2022, 225(10),1807-1810.
5. Sak B., Holubova N., Kvetonova D., Hlaskova L., Tinavska J., Kicia M., Zajączkowska Ż., Kvac M. Comparison of the concentration of Encephalitozoon cuniculi genotypes I and III in inflammatory foci under experimental conditions. J.Inflamm.Res. 2022,15, 2721-2730
6. Zajączkowska Ż., Bastyrova Brutowska A., Akutko K., Mcevoy J., Sak B., Hendrich A.B., Łukianowski B., Kvac M., Kicia M. Horse-specific cryptosporidium genotype in human with Crohn's disease and arthritis [research letter].  Emerg.Infect.Dis. 2022 Vol.28 no.6 s.1289-1291.
7. Sycz, Z; Wojnicz, D.; Tichaczek-Goska, D. Does secondary plant metabolite ursolic acid exhibit antibacterial activity against uropathogenic Escherichia coli living in single- and multispecies biofilms? Pharmaceutics  2022 Vol.14 no.8 art.1691

List of publications for 2021

1. Zajączkowska Ż., Akutko K., Kvac M., Sak B., Szydłowicz M., Hendrich A.B., Iwańczak B., Kicia M. Enterocytozoon bieneusi infects children with inflammatory bowel disease undergoing immunosuppressive treatment. Front. Med. (Lausanne) 2021, 8, art.741751.
2. Kaczmarek A., Sobociński W., Wesołowska M., Gołąb E., Kołodziej-Sobocińska M., Sałamatin R. Blastocystis occurrence and subtype diversity in wild European terrestrial mammals - the case of Białowieża Primeval Forest (NE Poland). Int.J.Parasitol.-Parasit. Wildl. 2021,16,120-125.
3. Jama-Kmiecik A., Sarowska J., Wojnicz D., Choroszy-Król I., Frej-Mądrzak M. Natural products and their potential anti-HAV activity. Pathogens 2021 Vol.10 no.9 art.1095
4. Wojnicz D., Tichaczek-Goska D., Gleńsk M., Hendrich  A.B, Is it worth combining Solidago virgaurea extract and antibiotics against uropathogenic Escherichia coli rods? An in vitro model study. Pharmaceutics 2021 Vol.13 no.4 art.573
5. Kvac M., Myskova E., Holubova N., Kellnerova K., Kicia M., Rajsky D., Mcevoy J., Feng Y., Haznal V., Sak B. Occurrence and genetic diversity of Cryptosporidium spp. in wild foxes, wolves, jackals, and bears in central Europe. Folia Parasitol. 2021 Vol.68 art.002
6. Ślusarczyk S., Cieślak A., Yanza YR., Szumacher-Strabel M., Varadyova Z., Stafiniak M., Wojnicz D., Matkowski A. Phytochemical profile and antioxidant activities of Coleus amboinicus Lour. Cultivated in Indonesia and Poland. Molecules 2021 Vol.26 no.10 art.2915
7. Sarowska J., Wojnicz D., Jama-Kmiecik A., Frej-Mądrzak M., Choroszy-Król I. Antiviral potential of plants against noroviruses. Molecules 2021 Vol.26 no.15 art.4669
8. Szydłowicz M., Matos O. Pneumocystis pneumonia in the COVID-19 pandemic era: similarities and challenges. Trends Parasitol. 2021 Vol.37 no.10 s.859-862
9. VeckovaT., Sak B., Samkova E., Holubova N., Kicia M., Zajączkowska Ż., Hlaskova L.,Kvetonova D., Kvac M. Raw goat's milk, fresh and soft cheeses as a potential source of Encephalitozoon cuniculi. Foodborne Pathog.Dis. 2021 Vol.18 no.9 s.661-667.
10. Sycz Z., Tichaczek-Goska D., Jezierska-Domaradzka A., Wojnicz D. Are uropathogenic bacteria living in multispecies biofilm susceptible to active plant ingredient—asiatic acid?" Biomolecules 2021, 11, 1754.
11. Kawa-Rygielska, J.;Adamenko, K.; Pietrzak, W.; Paszkot,J.; Głowacki, A.; Gasiński, A.; Leszczyński, P. The potential of traditional Norwegian KVEIK Yeast for brewing novel beer on the example of Foreign Extra Stout. Biomolecules 2021,11, 1778.
12. Tichaczek-Goska, D.; Gleńsk, M.; Wojnicz, D. The enhancement of the photodynamic therapy and ciprofloxacin activity against uropathogenic Escherichia coli strains by Polypodium vulgare rhizome aqueous extract. Pathogens 2021,10, 1544.

List of publications for 2020

1. Hendrich  A.B,  Strugała P, Dudra A, Kucharska A.Z,  Sokół-Łętowska A, Wojnicz D, Cisowska A, Sroka Z, Gabrielska J: Microbiological, antioxidant and lipoxygenase-1 inhibitory activities of fruit extracts of chosen Rosaceae family species. Adv.Clin.Exp.Med. 2020 Vol.29 no.2; s.215-224.
2. Wesołowska M, Zając-Pytrus H, Masny A, Pytrus W, Knysz B, Golab E, Sałamatin R: Onchocerca jakutensis ocular infection in Poland: a new vector-borne human health risk? Parasites Vectors 2020 Vol.13; art.61 [4 s.]
3. Kopacz Ż, Kvac M, Piesiak P, Szydłowicz M, Hendrich A.B, Sak B, Mcevoy J, Kicia M: Cryptosporidium baileyi pulmonary infection in immunocompetent woman with benign neoplasm.  Emerg.Infect.Dis. 2020 Vol.26 no.8 s.1958-1961.
4. Brdickova K,  Sak B,  Holubova N, Kvetonova D, Hlaskova L, Kicia M, Kopacz Ż, Kvac M: Encephalitozoon cuniculi genotype II concentrates in inflammation foci. J.Inflamm.Res. 2020 Vol.13 s.583-593.

List of publications for 2019

1. Kicia M, Szydłowicz M, Cebulski K, Jakuszko K, Piesiak P,  Kowal A,  Sak B, Krajewska M,  Hendrich AB,  Kvac M, Kopacz Ż: Symptomatic respiratory Encephalitozoon cuniculi infection in renal transplant recipients. Int.J.Infect.Dis. 2019; 79: 21-25.
2. Szydłowicz M, Jakuszko K, Szymczak A, Piesiak P, Kowal A, Kopacz Ż,  Wesołowska M, Lobo ML, Matos O, Hendrich A.B, Kicia M: Prevalence and genotyping of Pneumocystis jirovecii in renal transplant recipients - preliminary report. Parasitol.Res. 2019; 118(1):181-189.
3. Sak B, Vecková T, Brdíčková K, Smetana P, Hlásková L, Kicia M, Holubová N, McEvoy J, Kváč M: Experimental Encephalitozoon cuniculi Infection Acquired from Fermented Meat Products. Foodborne Pathog.Dis. 2019;16(6):394-398.
4. Tichaczek-Goska D, Wojnicz D, Symonowicz K, Ziółkowski P, Hendrich A.B: Photodynamic enhancement of the activity of antibiotics used in urinary tract infections. Lasers Med.Sci. 2019, 34(8):1547-1553.
5. Kopacz Ż, Kvac M, Karpiński P, Hendrich A.B, Sąsiadek M,M, Leszczyński P, Sak B, McEvoy J, Kicia M: The first evidence of Cryptosporidium meleagridis infection in a colon adenocarcinoma from an immunocompetent patient. Front.Cell.Infect.Microbiol. 2019 Vol.9; art.35 [5 s.]
6. Condlova S, Horcickova M, Havrdova N, Sak B, Hlaskova L, Perec-Matysiak A, Kicia M, McEvoy J, Kvac M: Diversity of Cryptosporidium spp. in Apodemus spp. in Europe. Eur.J.Protistol. 2019;69:1-13.
7. Gleńsk M, Tichaczek-Goska D, Środa-Pomianek K, Włodarczyk M,  Wesolowski CA, Wojnicz D: Differing antibacterial and antibiofilm properties of Polypodium vulgare L. rhizome aqueous extract and one of its purified active ingredients–osladin.J.Herb.Med. 2019 Vol.17-18; art.100261 [6 s.]

List of publications for 2018

1. Maria Wesołowska, Weronika Rymer, Marta Kicia, M. Popiołek.: Concurrent infection of a young tourist by hookworm and Strongyloides stercoralis during low budget travel in Southeast Asia. Helminthologia 2018; 55(2):166-172.
2. Sokulska M, Kicia M, Wesolowska M, Piesiak P, Kowal A, Lobo ML, Kopacz Z, Hendrich A.B, Matos O. Genotyping of Pneumocystis jirovecii in colonized patients with various pulmonary diseases. Med.Mycol. 2018; 56(7): 809-815
3. Kicia M, Sędzimirska M, Sak B, Kváč M, Wesołowska M, Hendrich A.B, Kopacz Ż. Respiratory microsporidiosis caused by Enterocytozoon bieneusi in a HIV-negative hematopoietic stem cell transplant recipient. Int.J.Infect.Dis. 2018; 77: 26-28.
4. Kicia M., Wesołowska M., Kopacz Z., Kváč M., Sak B., Sokulska M., Cebulski K., Hendrich A.B., Pozowski A. Disseminated infection of Encephalitozoon cuniculi associated with osteolysis of hip periprosthetic tissue. Clin. Infect. Dis. 2018; 67(8): 1228-1234.
5. Justyna Gil, David Ramsey, Paweł Pawłowski, Elżbieta Szmida, Przemysław Leszczyński, Marek Bębenek, Maria M. Sąsiadek.: The influence of tumor microenvironment on ATG4D gene expression in colorectal cancer patients
   Med.Oncol. 2018; 35(12); art.159 [8 s.].

List of publications for 2017

1. Alanio A, Gits-Muselli M, Guigue N, Desnos-Ollivier M, Calderon EJ, Di Cave D, Dupont D, Hamprecht A, Hauser PM, Helweg-Larsen J, Kicia M, Lagrou K, Lengerova M, Matos O, Melchers WJG, Morio F, Nevez G, Totet A, White LP, Bretagne S. Diversity of Pneumocystis jirovecii Across Europe: A Multicentre Observational Study. EBioMedicine 2017; 22:155-163.
2. Dorota Wojnicz, Dorota Tichaczek-Goska, Kamila Korzekwa, Marta Kicia, Andrzej Hendrich.: Anti-enterococcal activities of pentacyclic triterpenes. Adv.Clin.Exp.Med. 2017; 26(3):483-490.
3. Justyna Gil, David Ramsey, Elżbieta Szmida, Przemysław Leszczyński, Paweł Pawłowski, Marek Bębenek, Maria M. Sąsiadek.: The BAX gene as a candidate for negative autophagy-related genes regulator on mRNA levels in colorectal cancer. Med.Oncol. 2017; 34; art.16 [7 s.]
4. Jolanta Piekarska, Marta Kicia, Maria Wesołowska, Żaneta Kopacz, Michał Gorczykowski, Barbara Szczepankiewicz, Martin Kvac, Bohumil Sak.: Zoonotic microsporidia in dogs and cats in Poland.
   Vet.Parasitol. 2017; 246:108-111
5. Agnieszka Perec-Matysiak, Maria Wesołowska, Kinga Leśniańska, Katarzyna Buńkowska-Gawlik, Joanna Hildebrand, Marta Kicia.: Survey for zoonotic microsporidian pathogens in wild living urban rooks (Corvus frugilegus). J.Eukaryot.Microbiol. 2017; 64(5): 721-724
6. Martin Kvac, Lada Hofmannova, Ynes Ortega, Nikola Holubova, Michaela Horcickova, Marta Kicia, Lenka Hlaskova, Dana Kvetonova, Bohumil Sak, John McEvoy.: Stray cats are more frequently infected with zoonotic protists than pet cats. Folia.Parasitol. 2017; 64; art.034 

List of publications for 2016

1. Maria Wesołowska, Beata Szostakowska, Marta Kicia, Bohumil Sak, Martin Kvac, Brygida Knysz.: Cryptosporidium meleagridis infection: the first report in Poland of its occurrence in an HIV-positive woman. Ann.Parasitol. 2016; 62(3):239-241.
2. Marta Kicia, Maria Wesołowska,Żaneta Kopacz, Katarzyna Jakuszko, Bohumil Sak, D. Kvetonova, Magdalena Krajewska, M. Kvac.: Prevalence and molecular characteristics of urinary and intestinal microsporidia infections in renal transplant recipients. Clin.Microbiol.Infect. 2016; 22(5):462.e5-462.e9.
3. Dorota Wojnicz,Dorota TichaczekGoska,Kamila Korzekwa,Marta Kicia, Andrzej B. Hendrich.: Study of the impact of cranberry extract on the virulence factors and biofilm formation by Enterococcus faecalis strains isolated from urinary tract infections. Int.J.Food Sci. Nutr. 2016; 67(8):1005-1016.
4. Alicja Dolzblasz, Elwira Smakowska, Edyta M. Gola, Katarzyna Sokołowska, Marta Kicia, Hanna Janska.: The mitochondrial protease AtFTSH4 safeguards Arabidopsis shoot apical meristem function. Sci.Rep. 2016; vol.6; art.28315 [14s.]

List of publications for 2015

1. Wojnicz D, Tichaczek-Goska D, Kicia M. Pentacyclic triterpenes combined with ciprofloxacin help to eradicate the biofilm formed in vitro by Escherichia coli. Indian J.Med.Res. 2015; 141(3):343-353.
2. Elżbieta Szmida, Paweł Karpiński, Przemysław Leszczyński, Tomasz Sędziak, Wojciech Kielan, Paweł Ostasiewicz, Maria M. Sąsiadek.: Aberrant methylation of ERBB pathway genes in sporadic colorectal cancer. J.Appl.Genet. 2015 Vol.56 no.2; s.185-192
3. Sokulska M, Kicia M, Wesołowska M, Hendrich AB. Pneumocystis jirovecii – from a commensal to pathogen: clinical and diagnostic review, Parasit. Res. 2015; 114(10):3577-3585.
4. Paulina Strugała, Anna Dudra, Alicja Z. Kucharska, Anna Sokół-Łętowska, Dorota Wojnicz,Agnieszka Cisowska, Stefan Walkowski, Zbigniew Sroka, Janina Gabrielska, Andrzej B. Hendrich.: Biological activity of the methanol and water extracts of the fruits of anthocyanin-rich plants grown in South-West Poland. Nat.Prod.Commun. 2015;10(3):467-474

List of publications for 2014

1. Wesolowska M, Knysz B, Reich A, Blazejewska D, Czarnecki M, Gladysz A, Pozowski A, Misiuk-Hojlo M. Prevalence of Demodex spp. in eyelash follicles in different populations. Arch Med Sci. 2014; 12(10):319-24.
2. Kváč M, Saková K, Kvĕtoňová D, Kicia M, Wesołowska M, McEvoy J, Sak B. Gastroenteritis caused by the Cryptosporidium hedgehog genotype in an immunocompetent man. J Clin Microbiol. 2014; 52(1):347-9.
3. Kicia M, Wesolowska M, Jakuszko K, Kopacz Ż, Sak B, Květonova D, Krajewska M, Kváč M. Concurrent infection of the urinary tract with Encephalitozoon cuniculi and Enterocytozoon bieneusi in a renal transplant recipient. J Clin Microbiol. 2014 ;52(5):1780-2.
4. Agnieszka Stembalska, Przemysław Leszczyński, Justyna Gil, David Ramsey, Grzegorz Pitala, Adam Maciejczyk, Marcin Frączek.: Global DNA methylation status in laryngeal cancer. Head Neck 2014; 36(3):419-424
5. Agnieszka Cisowska, Gabriela Bugla-Płoskońska.: Analysis of the SDS-PAGE patterns of outer membrane proteins from Escherichia coli strains that have lost the ability to form K1 antigen and varied in the susceptibility to normal human serum. Folia Microbiol. 2014; 59(1):37-43

List of publications for 2013

1. Dorota Wojnicz, Dorota Tichaczek-Goska.: Effect of sub-minimum inhibitory concentrations of ciprofloxacin, amikacin and colistin on biofilm formation and virulence factors of Escherichia coli planktonic and biofilm forms isolated from human urine Braz.J.Microbiol. 2013 Vol.44 no.1; s.259-265
2. Dorota Wojnicz, Marta Kicia, Dorota Tichaczek-Goska.: Effect of asiatic and ursolic acids on morphology, hydrophobicity, and adhesion of UPECs to uroepithelial cells. Folia Microbiol. 2013 Vol.58 no.3; s.245-252
3. Przemysław Leszczyński, Andrzej Bogusław Hendrich, Elżbieta Szmida, Maria Małgorzata Sąsiadek.: ADAM and ADAMTS family proteins and their role in the colorectal cancer etiopathogenesis. BMB Rep. 2013 Vol.46 no.3; s.139-150
4. Dorota Wojnicz, Dorota Tichaczek-Goska, Marta Kicia.: Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains. Turk.J.Biol. 2013 Vol.37 no.5; s.556-564

SZCZEGÓŁOWY PLAN ZAJĘĆ Katedry w semestrze letnim 2023/2024

Schedule of the Department - Summer semester 2023/2024

TERMINY EGZAMINÓW - szczegóły w zakładkach dla poszczególnych kierunków

EXAMS TIMETABLE - MEDICINE - details in the tabs for the faculty of Medicine

EXAMS TIMETABLE - DENTISTRY - details in the tabs for the faculty of Dentistry

Molecular Biology

Course coordinator: Prof. Andrzej Hendrich

Semester: winter (I)
Lectures: 25 hours
Non-Clinical Classes (CN): 15 hours
Seminars: 25 hours
The form of completing the course: WRITTEN EXAM (test)

SYLLABUS: 2023/2024; 2022/2023; 2021/2022; 2020/2021

Egzamin - 2. termin poprawkowy - 6.03.2024 - WYNIKI

EGZAMIN I termin – 8.02.2024 - WYNIKI 

Egzamin - I termin poprawkowy – 29.02.2024 - WYNIKI

Osoby, które nie zdały egzaminu mogą obejrzeć swoje prace w poniedziałek 4.03.2024 w godz. 9:30-13:00, w Kat. Biologii na 2 piętrze

Egzamin - II termin poprawkowy – 6.03.2024 o  godz. 15:30 - s. 209 w CNIM, ul Marcinkowskiego 2-6.

KOLOKWIUM DOPUSZCZAJĄCE 1- przeprowadzane jest przez nauczyciela odpowiedzialnego za daną grupę; odbędzie się w ostatnim tygodniu semestru zimowego, jednak nie później niż 31.01.2024

KOLOKWIUM DOPUSZCZAJĄCE 2 - przeprowadzane jest przez nauczyciela odpowiedzialnego za daną grupę; odbędzie się 9.02.2024

CLASSES SCHEDULE

PROGRAMME OF SEMINARS, CLASSES AND LECTURES; books

SAFETY RULES during CLASSES – AVOIDING HAZARDS

INTERNAL REGULATIONS OF THE DEPARTMENT OF BIOLOGY 

Application for the subject credit – download

Parasitology workbook – mandatory in printed version - download

CONSULTATIONS - TIMETABLE HERE  (or by prior appointment with the teacher by e-mail)

ATTENTION!!!!
Application for subject (or part of the subject) exemption should be submitted to the office of the Department of Medical Biology and Parasitology, ul. Mikulicza-Radeckiego 9 (floor II), or by e-mail to malgorzata.pekalska-cisek@umw.edu.pl or dorota.tichaczek-goska@umw.edu.pl until 15.10.2023 !!!

Molecular Biology with Basics of Genetics

Course coordinator: Prof. Andrzej Hendrich

Semester: winter (I)
Lectures: 10 hours
Non-Clinical Classes (CN): 25 hours
The form of completing the course: WRITTEN EXAM (test)

SYLLABUS: 2023/2024; 2022/2023; 2021/2022; 2020/2021

Egzamin 2. termin poprawkowy - 6.03.2024 - WYNIKI

Egzamin I termin – 6.02.2024 - WYNIKI

Egzamin I termin poprawkowy – 28.02.2024 - WYNIKI

Osoby, które nie zdały egzaminu mogą obejrzeć swoje prace w poniedziałek 4.03.2024 w godz. 9:30-13:00, w Kat. Biologii na 2 piętrze

Egzamin II termin poprawkowy – 6.03.2024 o  godz. 15:30 - s. 209 w CNIM, ul. Marcinkowskiego 2-6.

KOLOKWIUM DOPUSZCZAJĄCE 1- przeprowadzane jest przez nauczyciela odpowiedzialnego za daną grupę; odbędzie się w ostatnim tygodniu semestru zimowego, jednak nie później niż 31.01.2024

KOLOKWIUM DOPUSZCZAJĄCE 2 - przeprowadzane jest przez nauczyciela odpowiedzialnego za daną grupę; odbędzie się 9.02.2024

CLASSES SCHEDULE

PROGRAMME OF CLASSES AND LECTURES; books

SAFETY RULES during CLASSES – AVOIDING HAZARDS

INTERNAL REGULATIONS OF THE DEPARTMENT OF BIOLOGY 

Application for the subject credit – download

Parasitology workbook – mandatory in printed version - download

CONSULTATIONS - TIMETABLE HERE (or by prior appointment with the teacher by e-mail)

ATTENTION!!!!
Application for subject (or part of the subject) exemption should be submitted to the office of the Department of Medical Biology and Parasitology, ul. Mikulicza-Radeckiego 9 (floor II), or by e-mail to malgorzata.pekalska-cisek@umw.edu.pl or dorota.tichaczek-goska@umw.edu.pl until 15.10.2023 !!!

Infectious Diseases – Parasitology

Classes: (3 hours)
Place: Department of Medical Biology and Parasitology; ul. Mikulicza-Radeckiego 9, ground floor lub floor II
Teachers:
Maria Wesołowska, PhD
Marta Kicia, PhD, Prof. of the Wroclaw Medical University
Żaneta Zajączkowska, PhD 

TIMETABLE OF CLASSES 2022/2023

PROGRAMME – content of classes:
Cosmopolitan parasites in the temperate climate zone, opportunistic parasites, selected parasitic infections imported from tropical countries, epidemiology.

Students are obliged to know: the risk of infection; routes of parasite transmission;
infective stages; the way and mode of leaving from human organisms by parasites; location of the parasites in the human body, and when the parasitic infection is associated with clinical risk; new infection and latent infection; geographic distribution; epidemiology and prophylaxis.
Credit with grade: colloquium

INTERNAL REGULATIONS OF THE DEPARTMENT OF BIOLOGY

SAFETY RULES – AVOIDING HAZARDS

CONSULTATIONS - schedule here by prior appointment with the teacher by e-mail

Parasitological Diagnostics

Course coordinator: Prof. Andrzej Hendrich

Semester: summer (IV)
Lectures: 30 hours
Non-Clinical Classes (CN): 30 hours
The form of completing the course: written exam (MCQ test)

SYLLABUS: 2022/2023; 2021/2022; 2020/2021

CLASSES SCHEDULE

PROGRAMME OF CLASSES AND LECTURES; books

SAFETY RULES during CLASSES – AVOIDING HAZARDS

INTERNAL REGULATIONS OF THE DEPARTMENT OF BIOLOGY

CONSULTATIONS – by prior appointment with the teacher by e-mail

Genetyka

Osoba odpowiedzialna za przedmiot: Prof. dr hab. Andrzej Hendrich

Semestr: letni (II)
Wykłady: 40 godz.
Ćwiczenia audytoryjne (CA): 10 godz.
Samokształcenie kierowane (SK): 20 godz.
Forma zakończenia przedmiotu: Kolokwium zaliczeniowe (MCQ)

SYLABUSY: 2023/2024

ADIUNKT DYDAKTYCZNY
dr Dorota Tichaczek-Goska
e-mail: dorota.tichaczek-goska@umw.edu.pl
tel. 71 784 15 23

PLAN ZAJĘĆ

PROGRAM ĆWICZEŃ I WYKŁADÓW; podręczniki - tryb stacjonarny

PROGRAM ĆWICZEŃ I WYKŁADÓW; podręczniki - tryb niestacjonarny

REGULAMIN WEWNĘTRZNY KATEDRY i ZAKŁADU BIOLOGII 

Podanie o zaliczenie przedmiotu - pobierz

KONSULTACJE - GRAFIK tutaj lub po wcześniejszym umówieniu się z nauczycielem drogą mailową

UWAGA!!!
Podanie o zwolnienie z przedmiotu można składać w sekretariacie Katedry i Zakładu Biologii i Parazytologii Lekarskiej, ul. Mikulicza-Radeckiego 9 (II piętro) lub drogą mailową na adres malgorzata.pekalska-cisek@umw.edu.pl lub dorota.tichaczek-goska@umw.edu.pl do 08.03.2024 !!!

Biologia molekularna

Osoba odpowiedzialna za przedmiot: Prof. dr hab. Andrzej Hendrich

Semestr: letni (II)
Wykłady: 30 godz.
Ćwiczenia niekliniczne (CN): 15 godz.
Seminaria: 30 godz.
Forma zakończenia przedmiotu: EGZAMIN PISEMNY (test MCQ)

SYLABUS: 2023/2024

ADIUNKT DYDAKTYCZNY
dr Dorota Tichaczek-Goska
e-mail: dorota.tichaczek-goska@umw.edu.pl
tel. 71 784 15 23

PLAN ZAJĘĆ

PROGRAM SEMINARIÓW, ĆWICZEŃ I WYKŁADÓW; podręczniki

ZASADY BHP PODCZAS ZAJĘĆ - UNIKANIE ZAGROŻEŃ

REGULAMIN WEWNĘTRZNY KATEDRY i ZAKŁADU BIOLOGII 

Podanie o zaliczenie przedmiotu - pobierz

Zeszyt ćwiczeń z parazytologii - obowiązkowy w wersji papierowej !!! - pobierz tutaj
- każdy student przynosi wydrukowany zeszyt ćwiczeń, ołówek i gumkę na pierwsze ćwiczenia z części parazytologicznej (gr. CN1A i CN1B - 1.03.2024; gr. CN2A i CN2B - 12.04.2024).

KONSULTACJE - po wcześniejszym umówieniu się z nauczycielem drogą mailową:

dr hab. Dorota Wojnicz - dorota.wojnicz@umw.edu.pl
dr hab. Maria Wesołowska - maria.wesolowska@umw.edu.pl
dr hab. Marta Kicia, prof. UMW - marta.kicia@umw.edu.pl
dr Dorota Tichaczek-Goska - dorota.tichaczek-goska@umw.edu.pl
dr Żaneta Zajączkowska - zaneta.kopacz@umw.edu.pl
mgr Błażej Łukianowski - blazej.lukianowski@umw.edu.pl

UWAGA!!!
Podanie o zwolnienie z przedmiotu (lub jego części) można składać w sekretariacie Katedry i Zakładu Biologii i Parazytologii Lekarskiej, ul. Mikulicza-Radeckiego 9 (II piętro) lub drogą mailową na adres malgorzata.pekalska-cisek@umw.edu.pl lub dorota.tichaczek-goska@umw.edu.pl do 08.03.2024 !!!

Molekularne Podstawy Diagnostyki Parazytologicznej

Koordynator przedmiotu:  dr hab. Marta Kicia, prof. UMW

Nauczyciele prowadzący zajęcia:
dr hab. Marta Kicia, prof. UMW
dr Przemysław Leszczyński
mgr Antonina Lewicka
mgr Błażej Łukianowski

Studenci: I-IV rok kierunek lekarski
Semestr: letni
Liczba godzin: 30
Rodzaj zajęć: ćwiczenia laboratoryjne (CL)

ADIUNKT DYDAKTYCZNY
dr Dorota Tichaczek-Goska
e-mail: dorota.tichaczek-goska@umw.edu.pl
tel. 71 784 15 23

PLAN ZAJĘĆ:  Grupa 1 - wtorki 14:00-16:15 - pierwsze zajęcia 5.03.2024
                           Grupa 2 - czwartki 14:00-16:15 - pierwsze zajęcia 7.03.2024

TEMATYKA ĆWICZEŃ:

Zasady rozpoznawania zarażeń pasożytami.
- W jakich przypadkach należy uwzględniać zarażenie pasożytami;
- Prawidłowy wywiad z pacjentem;
Podstawy molekularnych metod diagnostycznych.
- Poznanie podstawowych rodzajów molekularnych metod diagnostycznych;
- Zasady postępowania z materiałem biologicznym przeznaczonym do molekularnej diagnostyki parazytologicznej;
- Planowanie postępowania diagnostycznego w przypadku podejrzenia inwazji pasożytniczej;
Metodyka wykonania izolacji DNA; Projektowanie reakcji PCR;
 Zastosowanie molekularnych metod diagnostycznych w rozpoznawaniu zarażeń pasożytniczych.
- Przeprowadzenie izolacji DNA z różnych materiałów (kał, mocz, krew, płyn mózgowo-rdzeniowy, plwocina, zeskrobiny, bioptaty);
- Wykonanie reakcji PCR;
Zastosowanie molekularnych metod diagnostycznych w rozpoznawaniu zarażeń pasożytniczych c.d.
- Wykonanie rozdziału elektroforetycznego uzyskanych produktów;
- Wizualizacja i interpretacja wyników;
- Interpretacja wyniku badania laboratoryjnego;
Profilaktyka zarażeń pasożytniczych
- Najważniejsze zasady profilaktyki zarażeń pasożytniczych;

SYLABUS: 2022/2023; 2021/2022

KONSULTACJE 

Molecular Biology

Course coordinator: Prof. dr hab. Andrzej Hendrich

Semester: winter (I)
Lectures: 25 hours
Practicals: 15 hours
Seminars: 25 hours
The form of completing the course: WRITTEN EXAM (MCQ test)

TEACHING ASSISTANT PROFESSOR
Dr. Dorota Tichaczek-Goska
e-mail: dorota.tichaczek-goska@umw.edu.pl
tel. 71 784 15 23

FINAL EXAM

Final exam - 2nd re-take term - 6.03.2024  - RESULTS

The exam for students who received permission to reactivate it due to illness will take place on Thursday, March 14, 2024, at 2:00 p.m. at the Department of Biology and Medical Parasitology, 2nd floor.

Final exam - 1st term - 6.02.2024 at 11:00 a.m. - RESULTS

Final exam - 1st re-take term - 29.02.2024 at 11:45 a.m. - RESULTS

Students who did not pass the exam can view their works on Monday, March 4, 2024, at 9:30-13:00, in the Biology Department, 2nd floor

Final exam - 2nd re-take term - 6.03.2024 at 3:30 p.m. -  Library Lecture Hall - room 209, Marcinkowskiego 2-6

SUMMARIZING COLLOQUIUM 1 - will be held on Thursday, February 01, 2024, at 6:45 a.m. 
Details regarding which place a given student will take the test are HERE

SUMMARIZING COLLOQUIUM 1 - RESULTS

SUMMARIZING COLLOQUIUM 2 - will be held on Friday, February 09, 2024, at 10:00 a.m. in the Biology Department, in classrooms on the 2nd floor.

SYLLABUS:
2023/2024 (English version); 2023/2024 (Polish version);
2022/2023;
2021/2022;
2020/2021

SCHEDULE - timetable

COURSE PROGRAMM - the content of seminars, practicals and lectures; detailed schedule; books

SAFETY RULES during CLASSES

INTERNAL REGULATIONS of BIOLOGY DEPARTMENT

Application for the subject exemption - download

Parasitology - mandatory workbook (only printed/paper version !!!) - download

CONSULTATIONS - HERE or after e-mail contact with the teacher

ATTENTION!
Application for subject (or part of the subject) exemption should be submitted to the secretariat of the Biology Department, Mikulicza-Radeckiego 9 (2nd floor), or by e-mail to malgorzata.pekalska-cisek@umw.edu.pl or dorota.tichaczek-goska@umw.edu.pl until 15.10.2023 !!!

Molecular Biology with Basics of Genetics

Course coordinator: Prof. dr hab. Andrzej Hendrich

Semester: winter (I)
Lectures: 10 hours
Practicals: 25 hours
The form of completing the course: WRITTEN EXAM (MCQ test)

TEACHING ASSISTANT PROFESSOR
Dr. Dorota Tichaczek-Goska
e-mail: dorota.tichaczek-goska@umw.edu.pl
tel. 71 784 15 23

Final exam - 2nd re-take term - 6.03.2024  - RESULTS

Final exam - 1st term - 6.02.2024 - RESULTS

Final exam - 1st re-take term - 29.02.2024 at 11:45 a.m. - RESULTS

Final exam - 2nd re-take term - 6.03.2024 at 3:30 p.m. -  Library Lecture Hall - room 209, Marcinkowskiego 2-6

SUMMARIZING COLLOQUIUM 1 - will be held on Thursday, February 01, 2024, at 6:45 a.m. in the Biology Department

SUMMARIZING COLLOQUIUM 2 - will be held on Friday, February 09, 2024, at 10:00 a.m. in the Biology Department, in classrooms on the 2nd floor.

SUMMARIZING COLLOQUIUM 1 - RESULTS

SYLLABUS:
2023/2024 (English version); 2023/2024 (Polish version); 
2022/2023;
2021/2022;
2020/2021

SCHEDULE - timetable

COURSE PROGRAMM - the content of practicals and lectures; detailed schedule; books

SAFETY RULES during CLASSES

INTERNAL REGULATIONS of BIOLOGY DEPARTMENT

Application for the subject exemption - download

Parasitology - mandatory workbook (only in printed/paper version) - download

CONSULTATIONS - HERE or will be possible after e-mail contact with the teacher

ATTENTION!
Application for subject (or part of the subject) exemption should be submitted to the secretariat of the Biology Department, Mikulicza-Radeckiego 9 (2nd floor), or by e-mail to malgorzata.pekalska-cisek@umw.edu.pl or dorota.tichaczek-goska@umw.edu.pl until 15.10.2023 !!!

Infectious Diseases - Parasitology

Place: Department of Biology and Medical Parasitology, Mikulicza-Radeckiego 9, ground floor or second floor
Teacher: Dr hab. Maria Wesołowska
                 e-mail: maria.wesolowska@umw.edu.pl 

TEACHING ASSISTANT PROFESSOR
Dr. Dorota Tichaczek-Goska
e-mail: dorota.tichaczek-goska@umw.edu.pl
tel. 71 784 15 23

CONTENT of classes: Cosmopolitan parasites in the temperate climate zone, opportunistic parasites, selected parasitic infections imported from tropical countries, epidemiology.

Students are obliged to know the risk of infection, routes of parasite transmission, infective stages; the way and mode of leaving from human organisms by parasites, location of the parasites in the human body, and when the parasitic infection is associated with clinical risk; new infection and latent infection; geographic distribution; epidemiology and prophylaxis;
Form of credit: written colloquium (during second class)

SCHEDULE 2023/2024

ERASMUS dates of classes 2023/2024: 

• gr. CL1 - 05.12.2023 (Tuesday)  from 15:30 to 17:00 and 15.12.2023 (Friday) from 15:30 to16:15
• gr. CL2 - 09.01.2024  (Tuesday) from 15:30 to17:00 and 16.01.2024 (Tuesday) from15:45 to16:30
• gr. CL3 - 22.01.2024 (Monday) from14:00 to15:30 and 30.01.2024 (Tuesday) from11:15 to12:00

INTERNAL REGULATIONS of Biology Department

SAFETY RULES during CLASSES

CONSULTATIONS - HERE or after e-mail contact with the teacher

Molecular Basics of Parasitological Diagnostics

Course coordinator: Marta Kicia, PhD

Teachers:
Magdalena Szydłowicz, PhD
Przemysław Leszczyński, PhD
Antonina Lewicka, MA

Students: I-IV year Faculty of Medicine
Summer semester
Number of hours: 30
Type of classes: laboratory classes (CL)

SCHEDULE:

CONTENT of classes:

Principles for the diagnosis of parasitic infections.
- In which cases parasite infection should be considered;
- correct patient interview;
Basis of molecular diagnostics.
- Knowing basic types of molecular diagnostic methods;
- Principles of biological material management for molecular parasitological diagnosis;
Planning of diagnostic procedures in case of suspected parasitic infection;
Methodology of DNA isolation;
- PCR design;
Use of molecular diagnostic methods in the diagnosis of parasitic infections.
- DNA isolation from various materials (stool, urine, blood, CSF, sputum, biopsy);
- PCR; 
- Electrophoresis of obtained amplification products;
- Visualization and interpretation of results;
- Interpretation of laboratory tests results;
Prophylaxis of parasitic infections.
- The most important principles of parasitic infection prophylaxis;

SYLLABUS: 2022/2023; 2021/2022

CONSULTATIONS