Department of Medical Laboratory Diagnostics

In 1974, the Laboratory of Clinical Laboratory Diagnostics was established at the Biological and Pharmaceutical Institute of the Faculty of Pharmacy, which enabled fourth and fifth-year pharmacy students to specialise in clinical analytics. At that time, the head of the unit was Michał Masiak, PhD. The laboratory was located at 38 Szewska Street and the classes were conducted in the laboratories of university hospitals and in the room of the Division of Biochemistry of the Faculty of Pharmacy. In 1978, the Division of Medical Laboratory Diagnostics was created as part of the Institute of Bioanalysis and Environmental Research. Following the liquidation of the Institute in the academic year 1981/1982, the Department and Division of Medical Laboratory Diagnostics was created and the head was Prof. Michał Masiak, PhD. After the professor's death in 1993, the head was Stanisława Skowron-Szlósarczyk, PhD, and Prof. Mieczysław Woźniak, PhD, became the head of the Department and Division in 1995. In 2011, the Department of Medical Laboratory Diagnostics was created under the supervision of Prof. M. Woźniak, PhD. There were two divisions within the Department: the Division of Clinical Chemistry, headed by Iwona Bil-Lula, PhD, and the Division of Laboratory Haematology, the first head of which was Jadwiga Nowicka, PhD, and the duties were taken over by Iwona Urbanowicz, PhD, later on. In October 2019, two divisions were merged into one - Division of Clinical Chemistry and Laboratory Haematology and the head was Iwona Bil-Lula, PhD.
There are 17 employees in the Department: Iwona Bil-Lula, PhD, university professor, Grzegorz Sawicki, PHD, university professor, Assistant Professors: Mariola Śliwińska-Mossoń, PhD, Iwona Urbanowicz, PhD, Anna Krzywonos-Zawadzka, PhD, Wiesława Nahaczewska, PhD, Agnieszka Sapa-Wojciechowska, PhD, assistants: Agnieszka Olejnik, PhD, Jakub Szyller, PhD, Alina Rak-Pasikowska, MA, Marta Banaszkiewicz, MA, Aleksandra Franczak, MA, Katarzyna Wadowska, MA, engineering and technical employees: technicians: Renata Kozłowska, Agnieszka Bondyra, MA, and Magdalena Gil-Woźniak, MA - office assistant. It is worth mentioning that Henryk Owczarek, PhD, one of the main authors of the Laboratory Diagnostics Act and the first President of the National Chamber of Laboratory Diagnosticians in the period from 2002 to 2010, began his career in the Department.
The main areas of research of the Department are: the metabolism of cardiac tissue damaged by hypoxia and oxygen shock, the role of extracellular matrix metalloproteinases (MMPs) in the development of cardiovascular diseases, monitoring of the course of adenovirus infection in patients after haematopoietic cell transplantation and in obese patients, the role of the USP nuclear receptor in the regulation of gene expression, the factors influencing platelet ageing, the differences in the structure, reactivity and activity of platelet subpopulations, the influence of MMPs on thrombocyte function and the polymorphism of CDK inhibitor protein genes in CLL. As part of its scientific activity, the Department has also started international cooperation with many units, e.g.: Department of Pharmacology, University of Saskatchewan in Canada; School of Pharmacy and Pharmaceutical Sciences, University of Dublin, Ireland, and Center de Recherche en Cancérologie, Lyon, France.
As part of its didactic activity, the Department conducts classes for students in the Medical Analytics major for all years of study in the twelve core professional subjects. There are also scientific associations in the Department. The employees of the Department prepare textbooks for students and diagnosticians and translate foreign textbooks. The Department conducts specialisation courses for laboratory diagnosticians, e.g. in the fields of laboratory medical diagnostics and laboratory medical transfusiology, as well as development courses for diagnosticians and physicians. The practical part of the State Specialisation Examination for Laboratory Diagnosticians in laboratory medical diagnostics is organised in the Department.

• metabolism of cardiac tissue damaged by hypoxia and oxygen shock;
• the role of extracellular matrix metalloproteinases (MMPs) in the development of cardiovascular disease;
• the role of Klotho protein during ischemia-reperfusion injury of the heart;
• oxidative stress as an arrhythmogenic factor in healthy people and in cardiovascular diseases;
• pharmacological methods of protecting the kidney for transplantation against ischemia/reperfusion injury;
• monitoring the course of adenovirus infection in patients after hematopoietic cell transplantation and in obese patients;
• factors affecting the aging of platelets;
• differences in structure, reactivity and activity of platelet subpopulations; impact of MMP on thrombocyte functions;
• CDK inhibitory protein gene polymorphism in CLL;
• analysis of biochemical markers in the early diagnosis of lung cancer;
• the impact of genetic predisposition and environmental factors on the development of metabolic disorders and cancer.

Science projects 2014-2023:

1. Scientific research under the subsidy: The influence of selected pharmacological agents on the oxidation-reduction balance, activation of NLRP3 inflammasome and microvascular disorders as a result of ischemia and reperfusion. Task number: SUBK.D011.23.004 (2023)
2. Scientific research under the subsidy: Oxidative stress and its molecular effects as the basis of ventricular arrhythmias and a prelude to a new diagnostic algorithm. SUBK.D010.23.041 (2023)
3. Scientific research under the subsidy: The importance of extracellular matrix metalloproteinases in the ageing process of platelets in blood components for transfusion. SUBK.D010.23.005 (2023)
4. Scientific research under the subsidy: Analysis of polymorphisms of selected genes encoding cytochrome P450 enzymes metabolising tobacco-specific N-nitrosamines in the assessment of lung cancer risk.
    SUBK.D010.23.030 (2023)
5. Scientific research under the subsidy: The impact of Klotho protein on antioxidant and anti-apoptotic mechanisms in ischemic and reperfused heart tissue. Taks number: SUBK.D010.22.035 (2022)
6. Scientific research under the subsidy: The influence of antioxidants on the development of kidney damage in the metabolic syndrome. Task number: SUBK.D010.22.010 (2022)
7. Scientific research under the subsidy: Oxidative stress in the course of metabolic syndrome and other chronic diseases. Task number: SUBZ.D010.22.039 (2022)
8. Grant of the National Science Center: Evaluation of the correlation between polymorphisms of tumour necrosis factor-alpha and interleukin-6 genes and the risk of insulin resistance. Grant number: MINI.D010.21.007 (2022)
9. Grant of the National Science Center: Pharmacological protection of a transplanted kidney against ischemia-reperfusion injury. Grant number: UMO-2021/41/N/NZ3/01692 (2022-2025)
10. Scientific research under the subsidy: "Pharmacoprotection in cardiomyocyte damage caused by ischemia and reperfusion." Task number: SUB.D011.20.007 (2020)
11. Young Scientist Research Project 2020: "Biochemical markers in the diagnosis of early stage lung cancer - a preliminary study". Grant number: STM.D010.20.129 (2020)
12. Young Scientist Research Project: "Effect of doxycycline on platelets under conditions of chemical ischaemia and reperfusion induced in vitro" SIMPLE no. STM.D010.20.130. (2020)
13. Grant of the National Science Center: Protective effect of the Klotho protein on cardiomyocytes damaged as a result of acute ischemia and reperfusion. Grant number: UMO-2019/33/N/NZ3/01649 (2019-2023)
14. Grant of the National Science Center: Pharmacological methods of protecting the kidney for transplantation against ischemia/reperfusion injury - ex vivo and in vivo studies on an animal model. Grant number: UMO- 2017/27/B/NZ4/00601 (2018-2021)
15. Grant of the National Science Center: Evaluation of the effects of 5-phenoxyphenyl-5-alkylamino nitrates of barbituric acid on cardiac protection against ischaemia and reperfusion injury. grant number: UMO-2018/02/X/NZ4/02120 (2019)
16. Grant of the National Science Center: "Pharmacological suppression of the effects of acute ischemia with subsequent reperfusion to improve heart function. Ex vivo and in vivo studies". Grant number: UMO-2018/02/X/NZ4/02120 (2019)
17. Scientific research within the statutory activity: "N-substituted phenoxyphenyl derivatives of piperazine barbiturates as a potential cardioprotective factor during ischemia and reperfusion". Task number: ST-D.010.17.00.01 (2017-2018).
18. Grant of the National Science Center: "Regulation of metabolic processes of proteins of the contractile apparatus of the heart damaged as a result of oxygen shock" Grant number: 2014/15/B/NZ3/04865 (2015-2018).
19. Grant of the National Science Center: "Photoactive nanoliposome formulation as a strategy in photodynamic therapy of unstable atherosclerotic plaque". Grant number: GR/805/2014 (2014-2017)
20. Young Scientist Research Project: "Dipeptidyl peptidase IV as a new marker of metabolic syndrome". Grant number: Pbmn 174 (2013-2016)
21. Grant of the National Science Center: "Cardioprotective effect of synergism of inhibitors of post-translational modifications of myosin light chains (MLC1, MLC2) and matrix metalloproteinase 2 (MMP-2) inhibitors on the function of the I/R-injured heart". Grant number: UMO-2012/06/M/NZ3/00158 (2013-2016).
22. Grant of the National Center for Research and Development: "Pharmacoproteomic study of an isolated heart subjected to hypoxia and reperfusion". The “WROVASC—Integrated Center for Cardiovascular Medicine” project implemented under Measure 1.1 Support for scientific research for building a knowledge-based economy. Sub-measures 1.1.2 Strategic research and development programmes (POIG 01.01.02-02-001-/08-03) Task 23A (2013-2015).
23. Scientific research as part of the statutory activity: "Impact of adenoviral viremia on selected medical conditions". Task number: ST-947 (2015-2017).
24. Scientific research as part of the statutory activity: "Isolation of platelet subpopulations and their characteristics". Task number: ST-946 (2015-2017).
25. Scientific research as part of the statutory activity: "Monitoring of adenoviral therapy". Task number: ST-701 (2012-2014).
26. Scientific research as part of the statutory activity: "Platelet metalloproteinases". Task number: ST-702 (2012-2017).

List of publications (years 2018 - 2023):

1. P. Nowak, I. Bil-Lula, M. Śliwińska-Mossoń. A cross-talk about radioresistance in lung cancer - how to improve radiosensitivity according to chinese medicine and medicaments that commonly occur in pharmacies. International Journal of Molecular Sciences, 2023, vol. 24, no. 13, pp. art.11206 [20 p.]. DOI:10.3390/ijms241311206
2. M. Śliwińska-Mossoń, I. Bil-Lula, G. Marek. The cause and effect relationship of diabetes after acute pancreatitis. Biomedicines, 2023, vol. 11, no. 3, pp. art.667 [18 p.]. DOI:10.3390/biomedicines11030667
3. K. Wadowska, P. Błasiak, A. Rzechonek M. Śliwińska-Mossoń. Analysis of MMP-2-735C/T (rs2285053) and MMP-9-1562C/T (rs3918242) polymorphisms in the risk assessment of developing lung cancer, International Journal of Molecular Sciences, 2023, vol. 24, no. 13, pp. art.10576 [20 p.]. DOI:10.3390/ijms241310576
4. K. Wadowska, P. Błasiak, A. Rzechonek, I. Bil-Lula, M. Śliwińska-Mossoń. Hepcidin as a diagnostic biomarker in anaemic lung cancer patients, Cancers, 2023, vol. 15, no. 1, pp. art.224 [24 p.]. DOI:10.3390/cancers15010224
5. A. Radajewska, J. Szyller, J. Niewiadomska, A. Noszczyk-Nowak, I. Bil-Lula Punica granatum L. polyphenolic extract as an antioxidant to prevent kidney injury in metabolic syndrome rats, Oxidative Medicine and Cellular Longevity, 2023, vol. 2023, pp. art.6144967 [14 p.]. DOI:10.1155/2023/6144967
6. M. Banaszkiewicz, A. Krzywonos-Zawadzka, A. Olejnik, A. Noszczyk-Nowak, I. Bil-Lula Silencing RNA for MMPs may be utilized for cardioprotection, Cardiovascular Therapeutics, 2022, vol. 2022, pp. art.9729018 [12 p.]. DOI:10.1155/2022/9729018
7. M. Banaszkiewicz, P. Tarwacka, A. Krzywonos-Zawadzka A.Olejnik, R Laprairie, A. Noszczyk-Nowak, G Sawicki, I Bil-Lula. Δ9-Tetrahydrocannabinol (Δ9-THC) improves ischemia/reperfusion heart dysfunction and might serve as a cardioprotective agent in the future treatment.  Frontiers in Bioscience-Landmark, 2022, vol. 27, no 4,p. art.114 [16 p.]. DOI:10.31083/j.fbl2704114
8. P. Frydrychowski, M. Michałek, I. Bil-Lula, E. Chełmecka, A. Kafel, A. Noszczyk-Nowak, D. Stygar. Cardioprotective effect of acetylsalicylic acid in the myocardial ischemia-reperfusion model on oxidative stress markers levels in heart muscle and serum. Antioxidants, 2022, vol. 11, no 8, p. art.1432 [19 p.]. DOI:10.3390/antiox11081432
9. P. Frydrychowski, M. Michałek, W. Kuliczkowski, K. Nowak, P. Skrzypczak, I. Bil-Lula, A. Noszczyk-Nowak. The impact of a modified anaesthetic protocol on animal survival and the characteristics of ventricular arrhythmias in the course of acute myocardial infarction in a domestic pig model. Journal of Veterinary Research, 2022, vol. 66, no 3, p. 435-447. DOI:10.2478/jvetres-2022-0046
10. A. Olejnik, A. Krzywonos-Zawadzka, M. Kamińska I. Bil-Lula. Klotho protein decreases MMP-mediated degradation of contractile proteins during ischaemia/reperfusion injury to the cardiomyocytes. International Journal of Molecular Sciences, 2022, vol. 23, no 24, p. art.15450 [14 p.]. DOI:10.3390/ijms232415450
11. A. Radajewska, A. Krzywonos-Zawadzka, I. Bil-Lula. Recent methods of kidney storage and therapeutic possibilities of transplant kidney. Biomedicines, 2022, vol. 10, no 5, p. art.1013 [15 s.]. DOI:10.3390/biomedicines10051013
12. M. Skrzypiec-Spring, A. Sapa-Wojciechowska, A. Rak-Pasikowska, M. Kaczorowski, I. Bil-Lula, A. Hałoń, A. Szeląg. The protective effect of simvastatin on the systolic function of the heart in the model of acute ischemia and reperfusion is due to inhibition of the RhoA pathway and independent of reduction of MMP-2 activity. Biomolecules, 2022, vol. 12, no 9, p. art.1291 [13 p.]. DOI:10.3390/biom12091291
13. J. Szyller, D. Jagielski, I. Bil-Lula. Antioxidants in arrhythmia treatment - still a controversy? A review of selected clinical and laboratory research. Antioxidants, 2022, vol. 11, no 6, p. art.1109 [18 p.]. DOI:10.3390/antiox11061109
14. I. Urbanowicz, I. Bil-Lula. CAR-T- a new perspective on immunotherapy. Acta Poloniae Pharmaceutica, 2022, vol. 79, no 1, p. 3-15. DOI:10.32383/appdr/146852
15. B. Gajewska, M. Śliwińska-Mossoń Association of MMP-2 and MMP-9 polymorphisms with diabetes and pathogenesis of diabetic complications. International Journal of Molecular Sciences, 2022, vol. 23, no 18, p. art.10571 [12 p.]. DOI:10.3390/ijms231810571
16. M. Koral, J. Szyller, Polish experiences of pain treatment by paramedics in relation to good practices of pain treatment: a register-based study. Emergency Medicine International, 2022, vol. 2022, p. art.3677688 [9 p.]. DOI:10.1155/2022/3677688
17. J. Szyller, M. Kozakiewicz, P. Siermontowski, D. Kaczerska. Oxidative stress, HSP70/HSP90 and eNOS/iNOS serum levels in professional divers during hyperbaric exposition. Antioxidants, 2022, vol. 11, no 5, p. art.1008 [13 p.]. DOI:10.3390/antiox11051008
18. A. Olejnik, M. Banaszkiewicz, A. Krzywonos-Zawadzka, I. Bil-Lula.
    The Klotho protein supports redox balance and metabolic functions of cardiomyocytes during ischemia/reperfusion injury. Cardiology Journal, 2022, vol. 29, no 5, pp. 836-849. DOI: 10.5603/CJ.a2021.0174
19. M. Banaszkiewicz, A. Krzywonos-Zawadzka, A. Olejnik, A. Noszczyk-Nowak, I. Bil-Lula. Silencing RNA for MMPs May Be Utilized for Cardioprotection. Cardiovascular Therapeutics, 2022, vol. 2022, p. art.9729018. DOI: 10.1155/2022/9729018
20. M. Koral, J. Szyller. Polish Experiences of Pain Treatment by Paramedics in relation to Good Practices of Pain Treatment: A Register-Based Study. Emerg Med Int, vol. 2022, Article ID 3677688, 9 pages, 2022.
21. M. Banaszkiewicz, P. Tarwacka, A. Krzywonos-Zawadzka, A. Olejnik, R. Laprairie, A. Noszczyk-Nowak, G. Sawicki, I. Bil-Lula. Δ9-Tetrahydrocannabinol (Δ9-THC) improves ischemia/reperfusion heart dysfunction and might serve as a cardioprotective agent in the future treatment. Front.Biosci.Landmark 2022 vol.27 no.4 art.114 [16 p.], fig., bibliogr. item 82, summ. DOI: 10.31083/j.fbl2704114
22. M. Skrzypiec-Spring, J. Urbaniak, A. Sapa-Wojciechowska, J. Pietkiewicz, A. Orda, B. Karolko, R. Danielewicz, I. Bil-Lula, M. Woźniak, R. Schulz, A. Szeląg. Matrix metalloproteinase-2 inhibition in acute ischemia-reperfusion heart injury - cardioprotective properties of carvedilol. Pharmaceuticals 2021 Vol.14 no.12 art.1276 [15 p.]. DOI: 10.3390/ph14121276
23. I. Urbanowicz,  D. Wołowiec, B. Wysoczańska, P. Łacina, A. Jonkisz, W. Nahaczewska, A. Tukiendorf, I. Bil-Lula, K. Bogunia-Kubik, E. Pawlak. NF-κB1-94del/del ATTG polymorphic variant maintains CLL at an early, mildest stage.  Adv.Clin.Exp.Med. 2021 Vol.30 no.5 pp. 499-506. DOI: 10.17219/acem/128764
24. A. Franczak, R. Skomro, J. Sawicka, I. Bil-Lula, A. Nocon, M. Fenton, J. Lawson, G. Sawicki. Serum matrix metalloproteinase-2 as a predictor of level of hypoxemia and severity of obstructive sleep apnea.  Sleep Breath. 2021 Vol.25 no.2 pp.877-886. DOI: 10.1007/s11325-020-02200-3
25. K. Hałucha, A. Rak-Pasikowska, I. Bil-Lula. Protective role of platelets in myocardial infarction and ischemia/reperfusion injury. Cardiol.Res.Pract. 2021 Vol.2021 art.5545416 [14 p.]. DOI: 10.1155/2021/5545416
26. A. Franczak, R. Skomro, I. Bil-Lula, M. Fenton, J. Lawson, G. Sawicki. Activity of serum matrix metalloproteinase 9 in patients with obstructive sleep apnea.  Pol.Arch.Med.Wewn. 2021 Vol.131 no.6 pp.586-589. DOI: 10.20452/pamw.16014
27. M. Banaszkiewicz, A. Olejnik, A. Krzywonos-Zawadzka, K. Hałucha, I. Bil-Lula. Expression of atrial‑fetal light chains in cultured human cardiomyocytes after chemical ischemia‑reperfusion injury. Mol.Med.Rep. 2021 Vol.24 no.5 art.770 [10 p.]. DOI: 10.3892/mmr.2021.12410
28. M. Skrzypiec-Spring, A. Sapa-Wojciechowska, K. Haczkiewicz-Leśniak, T. Piasecki, J. Kwiatkowska, M. Podhorska-Okołów,  A. Szeląg. HMG-CoA Reductase Inhibitor, Simvastatin Is Effective in Decreasing Degree of Myocarditis by Inhibiting Metalloproteinases Activation. Biomolecules. 2021; vol. 11, no. 10: 1415. DOI: 10.3390/biom11101415.
29. K. Wadowska, P. Błasiak, A. Rzechonek, I. Bil-Lula, M. Śliwińska-Mossoń. New Insights on Old Biomarkers Involved in Tumor Microenvironment Changes and Their Diagnostic Relevance in Non-Small Cell Lung Carcinoma. Biomolecules. 2021; vol. 11 no. 8:1208. DOI: 10.3390/biom11081208.
30. Ł. Lewandowski, I. Urbanowicz, M. Kępińska, H. Milnerowicz. Concentration/activity of superoxide dismutase isozymes and the pro-/antioxidative status, in context of type 2 diabetes and selected single nucleotide polymorphisms (genes: INS, SOD1, SOD2, SOD3) - preliminary findings. Biomed.Pharmacother. 2021 Vol.137 art.111396 [20 p.]. DOI: 10.1016/j.biopha.2021.111396
31. I. Urbanowicz, D. Wołowiec, B. Wysoczańska, P. Łacina, A. Jonkisz, W. Nahaczewska, A. Tukiendorf, I. Bil-Lula, K. Boguniak-Kubik, E. Pawlak .NF-κB1-94del/del ATTG polymorphic variant maintains CLL at an early, mildest stage. Adv.Clin.Exp.Med. 2021 Vol.30 no.5 pp. 499-506. DOI: 10.17219/acem/128764
32. J. Szyller, I. Bil-Lula. Heat shock proteins in oxidative stress and ischemia/reperfusion injury and benefits from physical exercises: a review to the current knowledge. Oxidative Med.Cell.Longev. 2021 Vol.2021 art.6678457 [12 p.], fig. bibliogr. item 139, summ. DOI: 10.1155/2021/6678457
33. I. Urbanowicz, W. Nahaczewska, B. Celuch. Narrative review of aplastic anemia - the importance of supportive treatment. Ann.Pallliat.Med. 2021 Vol.10 no.1 pp.694-699, tab. bibliogr. item 35, summ. DOI: 10.21037/apm-20-1957
34. A. Olejnik, A. Krzywonos-Zawadzka, M. Banaszkiewicz, I. Bil-Lula. Ameliorating effect of Klotho protein on rat heart during I/R injury. Oxidative Med.Cell.Longev. 2020 Vol.2020 art.6427284 [11 p.], fig. tab. bibliogr. item 46, summ. DOI: 10.1155/2020/6427284
35. M. Moser, S. Schmid, K. Sawicka, T. Banerjee, E. McNair, J. Sawicka, I. Bil-Lula, G. Saiwcki. Pre-arrest doxycycline protects donation after circulatory death kidneys. Sci.Rep. 2020 Vol.10 art.22272 [9 p.], fig. bibliogr. item 19, summ. DOI: 10.1038/s41598-020-79440-6
36. M. Banaszkiewicz, A. Krzywonos-Zawadzka, A. Olejnik, I. Bil-Lula. Tissue expression of atrial and ventricular myosin light chains in the mechanism of adaptation to oxidative stress. Int.J.Mol.Sci. 2020 Vol.21 no.21 art.8384 [16 p.], fig. tab. bibliogr. item 45, summ. DOI: 10.3390/ijms21218384
37. M. Janik, S. Płaczkowska, M. Woźniak, I. Bil-Lula. Analysis of multiple risk factors for seronegative rate of anti-tick-borne encephalitis virus immunization in human serum. Medicina 2020 Vol.56 no.5 art.244 [12 p.], fig. tab. bibliogr. item 33, summ. DOI: 10.3390/medicina56050244
38. I. Urbanowicz. Aplazja szpiku (anemia aplastyczna) - aktualne rekomendacje. Jak wybrać najlepszą terapię? (Bone marrow aplasia (aplastic anemia) - current recommendations. How to choose the best therapy?) Nowa Hematol. 2020 pp. 55-63, tab. bibliogr. item 55,
39. A. Junka, M. Bartoszewicz, M. Dziadas, P. Szymczyk, K. Dydak, A. Żywicka, A. Owczarek, I. Bil-Lula, J. Czajkowska, K. Fijałkowski. Application of bacterial cellulose experimental dressings saturated with gentamycin for management of bone biofilm in vitro and ex vivo. J.Biomed.Mater.Res.Part B 2020 Vol.108 no.1 pp. 30-37, fig. bibliogr. item 25, summ. DOI: 10.1002/jbm.b.34362
40. A. Sapa-Wojciechowska, A. Rak – Pasikowska, K. Pormańczuk, B. Czapla, I. Bil-Lula. Extracellular matrix remodeling factors as markers of carotid artery atherosclerosis. Cardiol.Res.Pract. 2020 Vol.2020 art.9036157 [11 p.], fig. tab. bibliogr. item 53, summ. DOI: 10.1155/2020/9036157
41. K. Wadowska, I. Bil-Lula, Ł. Trembecki, M. Śliwińska-Mossoń.Genetic markers in lung cancer diagnosis: a review. Int.J.Mol.Sci. 2020 Vol.21 no.13 art.4569 [24 p.], fig. tab. bibliogr. 112 p. summ. DOI: 10.3390/ijms21134569
42. A. Olejnik, A. Krzywonos-Zawadzka, M. Banaszkiewicz, I. Bil-Lula. Klotho protein contributes to cardioprotection during ischaemia/reperfusion injury. J.Cell.Mol.Med. 2020 Vol.24 no.11 pp. 6448-6458, fig. tab. bibliogr. item 45, summ. DOI: 10.1111/jcmm.15293
43. M. Śliwińska-Mossoń, K. Wadowska, Ł. Trembecki, I. Bil-Lula. Markers useful in monitoring radiation-induced lung injury in lung cancer patients: a review. J.Pers.Med. 2020 Vol.10 no.3 art.72 [15 p.], tab. bibliogr. item 88, summ. DOI: 10.3390/jpm10030072
44. M.A.J. Moser, K. Sawicka, J. Sawicka, A. Franczak, A. Cohen, I. Bil-Lula, G. Sawicki. Protection of the transplant kidney during cold perfusion with doxycycline: proteomic analysis in a rat model. Proteome Sci. 2020 Vol.18 art.3 [12 p.], fig. tab. bibliogr. item 41, summ. DOI: 10.1186/s12953-020-00159-3
45. A. Krzywonos-Zawadzka, A. Franczak, G. Sawicki, I. Bil-Lula.: Mixture of MMP-2, MLC, and NOS inhibitors affects NO metabolism and protects heart from cardiac I/R injury. Cardiol.Res.Pract. 2020 Vol.2020; art.1561478 [10 p.]
46. A. Krzywonos-Zawadzka, M. Woźniak, G.Sawicki, I. Bil-Lula.: A drug cocktail for protecting against ischemia-reperfusion injury. Front. Biosci. Landmark 2020 Vol.25; p.722-735
47. A. Junka, M. Bartoszewicz, M. Dziadas, P. Szymczyk, K. Dydak, A. Żywicka, A. Owczarek, I. Bil-Lula, J. Czajkowska, K. Fijałkowski.: Application of bacterial cellulose experimental dressings saturated with gentamycin for management of bone biofilm in vitro and ex vivo. J. Biomed. Mater. Res. Part B 2020 Vol.108 no.1; pp. 30-37
48. W. Kuliczkowski, M. Banaszkiewicz, A. Mysiak, G. Makaś, I. Bil-Lula.: Does arterial hypertension affect plasma levels of matrix metalloproteinases and their tissue inhibitors in patients with stable coronary artery disease? A preliminary study. Cardiol. Res. Pract. 2019 Vol.2019; art.6921315 [8 p.]
49. A. Rak-Pasikowska, D. Goniwiecha, I. Bil-Lula.: Analiza błędów fazy przedanalitycznej w wybranych medycznych laboratoriach diagnostycznych (Analysis of pre-analytical phase errors in selected medical diagnostic laboratories). Diagn. Lab. 2019 Vol.55 no 2; p. 91-98
50. J. Nowicka, P. Milejski, I. Urbanowicz, P. Niewiński.: Antiviral immunity in chronic lymphocytic leukemia measured by anti-rubella antibody. Adv. Clin. Exp. Med. 2019 Vol.28 no.5; pp.679-682
51. A. Krzywonos-Zawadzka, A. Franczak, M. Moser, A. Olejnik, G. Sawicki, I. Bil-Lula.: Pharmacological protection of kidney grafts from cold perfusion-induced injury
    BioMed Res. Int. 2019 Vol.2019; art.9617087 [8 p.]
52. B. Celuch, I. Urbanowicz, J. Nowicka, W. Nahaczewska, I. Bil-Lula.: Zespoły mielodysplastyczne - współczesna diagnostyka (Myelodysplastic syndromes - contemporary diagnosis). Diagn. Lab. 2019 Vol.55 no 1; pp. 35-42
53. A. Krzywonos-Zawadzka, A. Franczak, A. Olejnik, M. Radomski, J. Gilmer, G. Sawicki, M. Woźniak, I. Bil-Lula.: Cardioprotective effect of MMP-2-inhibitor-NO-donor hybrid against ischaemia/reperfusion injury. J. Cell. Mol. Med. 2019 Vol.23 no.4; pp. 2835-2848
54. A. Franczak, I. Bil-Lula, G. Sawicki, M. Fenton, N. Ayas, R. Skomro.: Matrix metalloproteinases as possible biomarkers of obstructive sleep apnea severity - a systematic review. Sleep Med. Rev. 2019 Vol.46; pp. 9-16
55. I. Bil-Lula, M. Woźniak.: Co-infection with human polyomavirus BK enhances gene expression and replication of human adenovirus. Arch. Virol. 2018 Vol.163 no.7; pp.1841-1849
56. M. Wawrzyńska, I. Bil-Lula, A. Krzywonos-Zawadzka, J. Arkowski, M. Łukaszewicz, D. Hreniak, W. Stręk, G. Sawicki, M. Woźniak, M. Drab, K. Frączkowska, M. Duda, M. Kopaczyńska, H. Podbielska, D. Biały.: Biocompatible carbon-based coating as potential endovascular material for stent Surface. BioMed Res. Int. 2018 Vol.2018; art.2758347 [10 p.]
57. A. Olejnik, A. Franczak, A. Krzywonos-Zawadzka, M. Kałużna-Oleksy, I. Bil-Lula.: The biological role of klotho protein in the development of cardiovascular diseases. BioMed Res. Int. 2018 Vol.2018; art.5171945 [17 p.]
58. A. Olejnik, I. Bil-Lula, Ł. Kozera, A. Krzywonos-Zawadzka.: High serum concentration of dipeptidyl peptidase 4 at early stage of obesity - preliminary study. Diagn.Lab. 2018 Vol.54 no 4; pp. 233-239
59. P. Gliński, A. Rak-Pasikowska, A. Czapla, A. Sapa-Wojciechowska.: Użyteczność wybranych parametrów laboratoryjnych w diagnostyce udaru mózgu (The usefulness of selected laboratory parameters in the diagnosis of stroke). Diagn.Lab. 2018 Vol.54 no 4; pp. 275-284
60. D. Biały, M. Wawrzyńska, I. Bil-Lula, A. Krzywonos-Zawadzka, A. Sapa-Wojciechowska, J. Arkowski, M. Woźniak, G. Sawicki.: Low frequency electromagnetic field decreases ischemia-reperfusion injury of human cardiomyocytes and supports their metabolic function. Exp. Biol. Med. 2018 Vol.243 no.10; pp. 809-816
61. I. Bil-Lula, A. Krzywonos-Zawadzka, J. Sawicka, D. Biały, M. Wawrzyńska, M. Woźniak, G. Sawicki.: L-NAME improves doxycycline and ML-7 cardioprotection from oxidative stress. Front. Biosci. Landmark 2018 Vol.23 no.1; pp. 298-309.
62. M. Skrzypiec-Spring, K. Haczkiewicz, A. Sapa, T. Piasecki, J.Kwiatkowska, I. Ceremuga, M. Woźniak, W. Biczysko, Ch. Kobierzycki, P.Dzięgiel, M. Podhorska-Okołów, A. Szeląg.: Carvedilol inhibits matrix metalloproteinase-2 activation in experimental autoimmune myocarditis: possibilities of cardioprotective application. J.Cardiovasc.Pharmacol.Ther. 2018 Vol.23 no.1; pp.89-97
63. J. Nowicka, W. Nahaczewska, I. Urbanowicz, M. Woźniak.: Neopteryna jako wskaźnik stanu zapalnego i rozrostu nowotworowego w ostrych białaczkach (Neopterin as an indicator of inflammation and neoplastic growth in acute leukemias). Med. Rodz. 2018 R.21 no 1; pp. 27-33

BIOCHEMISTRY LABORATORY

• Bio-Rad Protean IEFCell bidirectional electrophoresis system
• Bio-Rad 1-DE electrophoresis equipment
• Bio-Rad wet transfer set
• Bio-Rad semi-dry transfer set
• CHEMI DOC BIORAD XRS plus imaging equipment
• Bio-Rad GS800KALIBRATET scanning densitometer
• SPARK TK BIOTECH multifunctional plate reader
• CHRONO-LOG two-channel aggregometer model 700

CARDIOMYOCYTE ISOLATION LABORATORY

• System for assessing cardiomyocyte contractility and calcium ion concentration in cells - IonOptix µStep System

HEART PERFUSION LABORATORY

• Emka Isolated Heart Perfusion (rodent heart perfusion device) using Langendorff method and working heart
• EMKA Isolated Kindey Perfusion (rodent kidney perfusion system)

HEART AND VASCULAR SYSTEM DOPPLER ULTRASONOGRAPHY LABORATORY

• Vevo 1100 ultrasound machine
• LEICA A60S stereo microscope

MOLECULAR BIOLOGY LABORATORY

• Spektrofotometr Nanodrop Lite Thermo Scientific
• Real Time PCR – CX96 Touch Real Bio-Rad

CELL CULTURE LABORATORY

• Diagnostic microscope with OPTA-TECH camera
• EVE AUTOMATIC CELLCOUNTER NANO ENTEK - cell counter

DIDACTIC LABORATORY

Haematology Laboratory

• Sysmex KX-21N hematology analyser
• 5-DIFF hematology analyser with MEK-8222 feeder
• Koagulometr CC-4000 bio-ksel

Clinical Chemistry Laboratory

• HPLC system with HP1000 detector
• A15 Biosystems automated analyser

Anna Krzywos-Zawadzka, PhD, is the Assistant Professor for Didactics at the Department
tel.: 71 784 06 24
e-mail: anna.krzywonos-zawadzka@umw.edu.pl

The Department conducts classes for students in the Medical Analytics major for all years of study in the twelve core professional subjects.

WINTER SEMESTER 2022/2023

MEDICAL ANALYTICS

YEAR I

1. Laboratory medicine in the health care system

YEAR III

1. Clinical Chemistry
2. General Analytics
3. Laboratory Hematology
4. Medical Law
5. Biological Material Collection Techniques

YEAR V

1. Laboratory Diagnostics
2. Paediatric Laboratory Diagnostics
3. Diagnosis of Diseases of Old Age

SUMMER SEMESTER 2022/2023

MEDICAL ANALYTICS

YEAR II
1. Clinical Chemistry
2. Organisation of Medical Diagnostic Laboratories
3. Quality and Accreditation Systems

YEAR III

1. Laboratory Hematology
2. Clinical Chemistry

YEAR IV

1. Blood Group Serology
2. Analytical Testing in Forensic Toxicology

The Department also offers optional classes for pharmacy and medical analytics students:

MEDICAL ANALYTICS

YEAR IV

1. Analytical testing in forensic toxicology
2. The extreme adventures of a globetrotter diagnostician - emergency and travel medicine
3.Tests for home use from the perspective of a diagnostician - advantages and risks
4.New psychoactive substances and their effects

PHARMACY

YEAR II

1. Hematology in a nutshell

YEAR IV

1. Analytical testing in forensic toxicology
2. New psychoactive substances and their effects

1. Scientific Association at the Heart Perfusion Laboratory (no. K165) - Iwona Bil-Lula, MD, PhD, university professor
2. Scientific Association, Laboratory Medicine (no. K192) - head Mariola Śliwińska-Mossoń, PhD

Wrocław Laboratory Medicine Meeting