Screening Biological Activity Assays and Collection of Biological Material Laboratory

Qsep 100 Bioptic capillary electrophoresis apparatus for DNA and RNA

• DNA separation range 20-15000bp

• analysis of up to 96 samples taken from a 96-well plate

Aspirator bottle FTA-2i

• for extracting alcohol or buffers remaining in tubes during DNA/RNA purification and other microparticle reprecipitation techniques

• it can also be used for washing cell cultures

• the bottle for the fluids to be suctioned is connected to the end part of the suction unit by a polyethylene tube. The fluid is removed when the suction tip comes into contact with the surface of the fluid

Autoclaves

a) Panasonic mls-3020U

b) Classic Prestige Medical (2 pieces - "dirty", "clean")

Automated cell counters

a) LUNA II YF Bioanalytic

• counting of unstained and stained cells

• possibility to check the correctness of cell counting

• possibility to set up an appropriate gate to count cells

• the device displays the number of counted cells and the percentage of live and dead cells

• storage of results possible via USB 3.0

b) TC20 Bio-Rad cell counter

• real-time view of the cells (the user can check if the counter identified the cells correctly)

• dilution calculator

• data export: Via USB stick

• cell concentration range: from 5x10^{^4} to 1x10^{^7} cells per millilitre

• optimal concentration range: from 1x10^{^5} to 5x10^{^6} cells per millilitre

• diameter of counted cells: 6-50 µm

• sample volume: 10 µl

Incubators

a) DH3600BII incubator

b) Panasonic MIR-162/262

Refrigerator-freezers, laboratory freezers (from +3°C to +16°C, from -9°C to -30°C)

CyFlow Space flow cytometer

• three air-cooled lasers

• blue 20mW 488nm,

• red 25mW 638nm or 635nm or 633nm

• and UV 16mW 375nm or 355nm

• 9 optical parameters: FSC, SSC, FL1 (FITC), FL2 (PE), FL3 (PE-Cy5), FL4 (PE-Cy7), FL5 (APC), FL6 (APC-Cy7), FL7 (CFP, Alexa 405)

• CCD camera

• Sorter

• particle size: 200nm - 60µm

• 15000 cells/s on input (maximum analysis speed)

• fraction purity 95% - on output 300 cells/s

• highest fraction purity 99% - on output 100 cells/s

• Different types of gating

ComboStik R700 urine analyser

• equipped with an internal printer

• stand-alone device (no PC required)

• capacity of 720 tests per hour

• parameters in the urine: blood, bilirubin, urobilinogen, ketones, proteins, nitrates, glucose, pH, specific gravity, leukocytes, microalbumin and creatinine (ComboStik 12MAC strips required)

Elisa Multiskan GO UV-Vis microplate reader, Thermo Scientific

• linear shaker with adjustable speed

• plates: 96- and 384-well (max. height: 18.5 mm)

• cuvettes: 12.5 x 12.5 x 40-58 [mm].

• wavelength range: 200-1000 nm (wavelength resolution - 1nm; wavelength accuracy - 2 nm; wavelength repeatability - 0.2 nm, read bandwidth <2.5 nm)

• reading range: up to 4 Abs

• spectral scanning speed: 10 s from 200 to 1000 nm with steps of 1 nm

Water deioniser
Makes it possible to obtain water of the third, second and first class of purity according to PN-EN ISO 3696:1999

• mechanical and carbon filters

• reverse osmosis (RO)

• demineralisation in a mixed ion exchange bed

• UV 254nm lamp

• microfiltration capsule

Densimat densitometer (bioMérieux)
Apparatus for measuring the density of a bacterial suspension in an ampoule
with a liquid substrate. Suspension density measurement can be performed for ampoules with distilled water, sodium chloride solution or liquid media.
Real-time measurement, with a range of 0 - 7.5 McFarland.

Printers and readers for self-adhesive alphanumeric/character codes/2D (the range from -196°C)

Fume cupboards

a) SWC-1A Dreamer

• suitable for all kinds of chemicals

b) fume cupboard with EX elements model S

• suitable for compounds where there is a risk of explosion

Omni TH hand-held homogenizer for soft and hard tissues

Incubators

a) CO₂ incubator Panasonic MCO-170AICVH-PE

b) CO2 incubator with hypoxia In-Vitro Cell NU 5841E

c) CO₂ incubator Eppendorf Galaxy 48 R

Laminar flow cabinets

• Laminar flow cabinet, safety class II, for working with cytotoxic substances SCANLAF Mars Pro Cytosafe Class 2

• Laminar flow cabinet, safety class II, SCANLAF Class 2

• ESCO laminar flow cabinet

• Biohazard NU-480-400E laminar flow cabinet

PORKKA KF85 ice machine

• water-cooled, with a stainless steel housing, with a capacity of 22 kg of ice per day (the container holds 6.5 kg of ice)

• the ice machine produces crushed ice

Shaking water baths

• operating range from +5°C above ambient temperature to 100°C

• flask holder platforms and test tube racks

Microscopes

a) microscope with camera and image analysis kit Olympus BCX43

• bright-field lenses (10x, 20x, 40x and 100x)

• five-position condenser for observation:

• in bright field

• phase contrast

• dark field

b) Olympus CKX41 inverted microscope

• phase-contrast and brightfield lenses (10x, 20x, 40x)

c) Olympus IX53 fluorescence microscope

• bright field and phase-contrast observations and Blue and Green light-induced fluorescence

• carousel for eight fluorescent filters

• long-distance phase objectives with 10x, 20x, 40x magnification

• fluorescent light source - mercury burner

d) Carl Zeiss Primo Vert microscope with camera

• gives the possibility to work with unstained cells by phase contrast and GFP-labeled cells by contrast

• universal phase contrast slider for all lenses - it is possible to use the Ph1 ring for 10x, 20x and 40x lenses and eliminate the need to switch the slider when changing magnification

• the camera enables looking at the image on the monitor screen and taking photos

Stirrers

a) Magnetic stirrer MST (VELP)
up to 1100 rpm

b) Magnetic stirrer with heating and a stand (WIGO)
50 - 1000 rpm
, temp. 150-160°C
two-piece tripod 400 mm

c) IKA MTS 2/4 orbital shaker
for titer plates, amplitude 3 mm, speed range 0 - 1100 rpm, holders for 2 or 4 plates.

Millicell ERS-2 and two electrodes MERSSTX01 and MERSSTX03 from Merck
A trans epithelial electrical resistance (TEER) measurement device for measuring the electrical resistance above and below the cell monolayer.

EMAG Emmi 20 HC ultrasonic cleaner
The Emmi 20HC two-litre all-purpose cleaner with adjustable ultrasonic power, temperature and a wide range of timers with an additional option for continuous operation.
The cleaner has a steel lid and a basket with handles, which makes it possible to place it in and remove it conveniently.
The ultrasonic power regulator enables achieving optimal efficiency for different types of contamination.

Thermocyclers

a) Toptical Gradient 96, Biometra

• lid temperature: 30 - 99°C

• temperature gradient: 40°C

• average heating rate: 5°C (max. 6°C)

• average cooling rate: 3.5°C (max. 4.5°C)

• temperature range 3 - 99°C

• temperature increment: min. 0.1°C/cycle

• time increment: min. 1 s/cycle

• data analysis: quantitative, relative without standard curve, ΔΔCt + curve determination for validation experiment, denaturation of amplicon in the presence of intercalating dye, genotyping

b) PCR C1000 Bio-Rad gradient thermal cycler

• holds: 2 x 48 x 0.2 ml tubes or two 48-well plates

• capacity:

• temperature range: 0-100°C

• maximum temperature increment: up to 5°C per second

• accuracy: ±0.2°C

• temperature uniformity between wells: ±0.4°C within 10 seconds

• Real-time PCR compatible: upgrades to CFX96 Touch™, CFX96 Touch Deep Well or CFX384 Touch™ Real-Time PCR Detection System

Titrando pH meter with an electrode (Metrohm)

• measurement range: -8,000…+22,000

• resolution: 0.001 pH

• accuracy: ±0.003 pH

• temperature: -150°C…+250°C

• potential: -1200mV…+1200mV

CoolCell FTS30 progressive freezing container for 2 ml biological samples

Isothermal containers for transporting biological material (10 L, 13 L, 20 L, 42 L)

Temperature recorders (1-channel in the ranges -30˚C to +70˚C, -80˚C to +70˚C, -100˚C to +70˚C, -200˚C to +70˚C, 5- channel in the range -200˚C to +50˚C)

Shimadzu PPSQ33A protein sequencer

Automatic determination of primary structure of proteins by Edman degradation, reversed-phase separation by high-performance liquid chromatography (difference in retention times) and detection of PTH-amino acids by absorbance at specific wavelengths, and identification and quantification by analysis of a standard of known concentration, comparison of retention times and UV absorbance.

Necessary reagents:

• 5% solution of phenyl isothiocyanate in n-heptane

• 12% trimethylamine solution

• 25% trifluoroacetic acid solution

• ethyl acetate

• 1-chlorobutane

• 37% acetonitrile solution

• the device requires nitrogen with a purity of min. 99.9999%

Gel documentation system

a) Bio-Rad ChemiDoc XRS+ imaging and documentation device for gels and membranes

• Image Lab Software

• possible applications: chemiluminescence, fluorescence, colourimetry/densitometry, gel documentation

• maximum gel size 28 x 36 cm

• maximum imaging size 26 x 35 cm (25 x 26 cm for UV-induced gels)

• 16-bit cooled CCD camera with auto-focus

• excitation source: EPI-white and trans-UV (302 nm)

• conversion screen for gels stained with Coomassie, silver and other gel dyes

• possibility to work with pre-loaded, ready-to-use imaging protocols

• possibility to save and restore the work protocols

b) G-Box Syngene

Spectrophotometer for determination of concentration and purity of nucleic acids and proteins in micro volumes NanoDrop Lite Plus

• 1-2 μL sample volume required

• stand-alone device (no PC required), touch screen

• measurement of dsDNA up to 1500 ng/μL, RNA up to 1200 ng/μL, BS up to 45 mg/mL

• measurement of A260/A280 and A260/A230

• possibility of exporting data directly to USB

Bio-Rad Trans-Blot Turbo rapid protein transfer system

• fast protein transfer - in 3 minutes (mini or midi gels)

• possibility of simultaneous transfer in one cycle: 1-4 mini gels or 1-2 midi gels

• customisable transfer conditions

Heating and cooling thermomixer - HLC DITABIS MKR13

• temperature range: -18°C to +105°C

• average block heating time: 25°C - 90°C: 5°C/min

• average block cooling time: 90°C - 25°C: 6.4°C/min

• 25°C - 10°C: 1.5°C/min

• shaking speed: 200 - 1500 rpm

• orbit: 3mm

Elmi thermostatic shaker

• capacity of four 96-well standard microplates at the same time

• thermostatic temperature range - from ambient temperature to 60°C

• thermostatic temperature setting with 0.1°C resolution

• difference between the set temperature and the actual temperature: ±0.1°C

• maximum time to reach the desired thermostatic temperature: up to 15 minutes

• programmable shaking frequency: from 100 to 1300 min-1

• orbit of the shaking platform with plates: 1.5 mm

• rotational shaking motion of the platform

• programmable working time of 1...999 minutes or switching to continuous operation

Smartcheck pipette verification device with PipetteX software

• the SmartCheck weighs the supplied volumetric samples using the gravimetric method, then calculates random and systematic error based on four readings. This makes it possible to obtain a quick positive/negative result for the device and the user, indicated by a green or red light.

• PipetteX is pipette management software. PipetteX software automatically stores pipette manufacturer information, notifies users when pipettes are due to be calibrated, and saves service data. This includes calibration reports, scheduled routine test periods and all routine check data for the SmartCheck or scales.

• possibility to control pipettes of any brand, including multi-channel pipettes, by testing individual channels

• possibility to check pipettes in the range of 100μl - 1000 μl

In vitro cell stretching device (Flexcell Tension System) FX - 5000 T

Laboratory centrifuges

a) Multifuge X1R with a refrigeration system

• - 90° tilt rotor; 4 x 180 ml; max. 275 g; 5500 rpm

• temperature range: 10 - 40°C

• rapid chill function

• 99 programmes (5 with direct access)

• password-protected programme access and lid opening option

• 9 acceleration curves + 10 deceleration curves

• time control range up to 99 hrs. 59 min. + continuous operation; short spin option

b) MPV-352R with a refrigeration system

• angular rotors for 2/1.5 ml Eppendorf type tubes (12, 24, 36 pieces) with lid; 18000 rpm -

• angular rotor for 8 x 50 ml Falcon type tubes with reduction inserts for 15 ml tubes; max. 5500 rpm

• horizontal rotor with containers and reduction inserts 4 x 250 ml

• temperature range: -20°C to +40°C

• rapid chill function

• 99 programmes

• 10 acceleration and 10 deceleration curves

• working time: 0-99 hrs., 0-59 min., 59 s + continuous operation; short spin option

c) MPV-260R with a refrigeration system

• angular rotors for 2/1.5 ml Eppendorf type tubes (12, 24, 36 pieces) with lid; 18000 rpm, 15100 rpm, 14000 rpm

• angular rotor for 6 x 50 ml Falcon type tubes with reduction inserts for 15 ml tubes; max. 6000 rpm

• temperature range: -20°C to +40°C

• rapid chill function

• 99 programmes

• 10 acceleration and 10 deceleration curves

• working time: 0-99 hrs., 0-59 min., 59 s + continuous operation; short spin option

d) Centrifuge 5702 without refrigeration system Eppendorf + swing-out rotor A-4-38 + adapters

• maximum speed: 3 000 × g (4 400 rpm)

• speed 100-4400 rpm (100 rpm steps)

• maximum capacity 4x100 ml

• acceleration and deceleration time <19s

• SOFT braking function for gentle acceleration and deceleration

• optimisation for cell separation by gradient centrifugation

• to ensure repeatability of centrifugation, the At set rpm function only starts the clock when the machine reaches the selected speed

• electronic unbalance detection for maximum safety

e) CAPP Rondo CR-68:

• maximum speed: 6000 rpm

• maximum RCF: 2000 x g

• replaceable rotors:

• for 2/1.5 ml tubes (8 pcs.)

• for 2 x 8-strip PCR tubes

• individual adapters for 0.4/0.5 ml tubes (8 pcs.)

• individual adapters for 0.2 ml tubes (8 pcs.)

f) Labnet MPS-1000 Mini Plate Spinner

• capacity: 2 PCR plates

• maximum speed: 2500 rpm

• max RCF: 500 x g

• 96 and 384-well plates

• possible centrifugation of half the plate, 8 or 12 x 0.2µl samples and a single PCR tube

Gel electrophoresis kit + power supply unit (C.B.S. SCIENTIFIC)

• adjustable tray for pouring gels

• 4 trays of the following sizes:

• 14cmx8cm

• 14cmx12cm

• 14cmx16cm

• 14cmx20cm

• two combs of 16 wells (max sample volume 101µl)

Bio-Rad vertical electrophoresis and western blotting kit

The kit includes:

1. Power supply unit

2. Items needed for the preparation of polyacrylamide gel (1mm and 1.5mm glasses, combs, stand)

3. An insert for transferring the gel onto the membrane (sponges, inserts...)

Vortexes

a) neoLAB D-6012

• NeoVortex with infinitely variable speed from 0 to 2500 rpm

• possibility to select the Press-to-Mix function

• circular movement along a 4 mm orbit

• vibration absorption thanks to specially shaped silicone feet and a steel base

• die-cast housing

b) Vortex ZX3

• continuous operation option, also activated and deactivated by touch

• adjustable speed - up to 3,000 rpm

c) Reax top

• movement type: eccentric

• shaking frequency: 0-2400 rpm

• orbit: 5 mm

• operating mode: intermittent and continuous

Precision balance ME2002/M (Mettler-Toledo AG)
up to 2200g, accurate to 0.01g

Analytical balance ME204/M (Mettler-Toledo AG)
up to 220g, accurate to 0.1mg

Low-temperature freezers (-40°C to -86°C)

Containers:

• for the transport and storage of liquid nitrogen (1 L, 10 L)

• for the storage of biological samples in liquid nitrogen/liquid nitrogen vapour (65 L, 60 L)

• for the transport of biological samples in liquid nitrogen vapour (25 L)

SOFTWARE:

• GraphPad Prism ver. 9.

The Screening Biological Activity Assays and Collection of Biological Material Laboratory has an extensive library of analyses, from popular to highly specific t-tests, one-, two- and three-way ANOVA, linear and non-linear regression, dose-response curves, binary logistic regression, survival analysis, principal component analysis and many more. Each analysis comes with a checklist to help the user understand the required statistical assumptions and confirm the appropriate test selection.

To register for measurements, use the software available in the Laboratory, report the need for access to the deposited biological material, please use the dedicated application available at https://app.umw.edu.pl/rezerwacja-aparatury/

You are also welcome to use the software available in the Laboratory, details and description under the Apparatus and Software tab.

PHARMACY MAJOR
ABC of Biobanking (elective course) - year II, Pharmacy
ABC of Biobanking (elective course) - year III, Pharmacy
New Perspectives for Biomedicine (elective course) - year I, Pharmacy

KIERUNEK ANALITYKA MEDYCZNA
Biomedycyna- najnowsze kierunki rozwoju (fakultet) - V rok Analityki medycznej
Podstawy biobankowania - V rok Analityki medycznej

KIERUNEK DIETETYKA
Biologia medyczna (wykład) - I rok Dietetyki

KONSULTACJE DLA STUDENTÓW
Poniedziałek 9:00 - 10:00