Department of Molecular and Cellular Biology

CONSULTATIONS:
To save your time and for us to be able to carry out the consultation more efficiently, please contact us in advance by e-mail or over the phone to arrange the consultation and to present its subject.https://www.umw.edu.pl/pl/jednostki/katedra-i-zaklad-biologii-molekularnej-i-komorkowejhttps://www.umw.edu.pl/pl/jednostki/katedra-i-zaklad-biologii-molekularnej-i-komorkowej

Prof. Jolanta Saczko, PhD - Tuesday 10:30-11:15
 Julita Kulbacka, PhD, MSc - Tuesday 10:30-11:15
Anna Choromańska, Phd, MSc - Tuesday 10:30-11:15
 Dagmara Baczyńska, PhD - Tuesday14:15-15:00
Nina Rembiałkowska, PhD - Tuesday 10:30-11:15
 Agnieszka Chwiłkowska, PhD - Tuesday 13:15-14:00
 Zofia Łapińska, MA - Tuesday 9:00-9:45
 Urszula Szwedowicz, MA - Tuesday 9:00-9:45
Agnieszka Gajewska-Naryniecka, MA - Tuesday 10:30-11:15

Persons who, due to their health condition, disability or other objective reasons, may require special assistance while attending classes, getting credits or preparing materials, are asked to present their personal situation using an e-mail or in person to the assistant professor for didactics or the person in charge of the classes.
We would like everyone to have equal rights to acquire knowledge and gain credits during the classes conducted by us.
Persons who are particularly entitled to report such needs are:
1.       Persons with various disabilities, in particular: mobility impaired, blind and partially sighted, deaf and hard of hearing, with mental disorders or illnesses, autistic persons;
2.       Persons with chronic diseases,
3.       Persons going through health crisis: mental, trauma, surgery

Pharmacy

Genetics year I summer semester

Results of the exam in Genetics, first date, on 28 June 2023.

To pass the exam in Genetics, it was necessary to get 60% of the points, which was 29 out of 48 points.

It will be possible to see the works on Monday, 3 July 2023, from 12:00 to 13:00 at the office.

Molecular biology year II winter semester

Elective course Cell death - how to die in several ways? Year I summer semester
Dates of elective classes: 22 May - 19 June 2023 (Monday) from 8:30 to 11:30, classroom C2/2/017
Materials for the preparation of the presentations will be made available on the university drive, and the link can be found in the university materials.

Elective course Molecular and cellular aspects of modern diagnostic methods
Year III winter semester

Medical analytics

Molecular Biology Year III summer semester

The link to the Molecular Biology lectures can be found in the university materials.
Seminars are held in the Seminar Room C2/2/017. Materials for the preparation of the presentations will be made available on the university drive, and the link can be found in the university materials.
Molecular Biology classes take place in the Exercise Room C1/5/001 according to the timetable below.
Before the classes, please read the instructions below, print them and bring them to the classes. Instructions for Practice Problems no 6 and 6, please print in colour.

Molecular Diagnostics Year IV summer semester

The link to the Molecular Diagnostics lectures can be found in the university materials.
Seminars are held in the Seminar Room C2/2/017. Materials for the preparation of the presentations will be made available on the university drive, and the link can be found in the university materials.
Classes take place in the Exercise Room C1/5/001 according to the timetable below.
Before the classes, please read the instructions below, print them and bring them to the classes.

Elective course Molecular and cellular aspects of modern diagnostic methods
 Year III winter semester

 Elective course Cell death - how to die in several ways?
Year III winter semester

Elective course In vitro methods in the laboratory
 Year IV summer semester
Dates of elective classes: 23 May - 20 June 2023 (Tuesday) from 13:00 to 16:00, room C2/2/017
 Materials for the preparation of the presentations will be made available on the university drive, and the link can be found in the university materials.

Medical Biology / the use of biological sciences in medicine
Medical Biology classes take place in the Exercise Room C1/5/001. Before the classes, please read the instructions below, print them and bring them to the classes.
The seminars in Medical Biology will start once the lectures are completed. Materials for the preparation of the presentations will be made available on the university drive, and the link will be made available in the university materials.
The classes timetable can be found below.

Genetics
The seminars is Genetics will start once the lectures are completed, according to the timetable below.  Materials for the preparation of the presentations will be made available on the university drive, and the link will be made available in the university materials.

Immunologia
II rok semestr letni

Fakultet Molekularne i komórkowe aspekty nowoczesnych metod diagnostycznych 
II rok semestr zimowy
Terminy fakultetów:  18.10 (śr) 12.00-15.00,  23.10 (pon) 10.30- 13.30,  25.10 (śr) 12.00-15.00, 6.11 (pon) 10.30-13.30, 8.11 (śr) 12.00-15.00 , fakultety będą obywały się on-line na platformie Teams
Materiały do przygotowania prezentacji zostaną udostępnione na dysku uczelnianym, a link został wysłany Staroście Roku

Dietetics level II

Year I winter semester

Elective course In vitro methods in the laboratory
Dates of elective classes: 10 November 2022 - 15 December 2022 (Thursday) from 17:15 to 20:15, room C2/2/017
Materials for the preparation of the presentations will be made available on the university drive, and the link will be made available inhttps://materialy.umw.edu.pl/ the university materials.

Fakultet Metody in vitro w laboratorium
I rok semestr letni

Fakultety będą odbywać się w sali seminaryjnej C2/2/017 w poniedziałki  8.04-6.05.2024  godz.14.15 - 17.15.

Materiały do przygotowania prezentacji zostaną udostępnione na dysku uczelnianym, a link znajduje się w Centralnym Repozytorium Materiałów Dydaktycznych.

1.Charakterystyka i typy hodowli komórkowych; środowisko hodowlane, media i suplementy, rozdzielanie i metody identyfikowania komórek. 
2.Skąd się wzięły hodowle? Klasyfikacja hodowli komórek i tkanek. Zastosowanie modelu komórkowego in vitro w nauce, biotechnologii, medycynie i przemyśle. 
3.Metody zliczania komórek  i analizy  typów śmierci komórkowej  – od metod podstawowych do automatycznych.
4.Linie komórkowe jako model badawczy funkcji genów. Przygotowanie komórek do podstawowych badań biologii molekularnej na poziomie DNA, RNA i białka.
5.Podwójne oblicze komórek macierzystych.
6.Komórki jako model doświadczalny w walidacji leków (test MTT, SRB i klonogenny, IC50, SI - interakcje).
7.Metody obrazowania kultur in vitro - 2D i 3D.
8.Co możemy wydrukować? - Bioprinting

Research directions:

- support of the transport of drugs by means of electroporation and sonoporation
 - microRNA and gene expression analysis
 - oxidative stress markers and assessment of free radical processes in anticancer therapies
 - multidrug resistance
- photodynamic therapy, its modulations and study of mechanisms
 - natural anti-cancer substances (curcumin, cavain, biochanin, catechin, etc.)
- the influence of radiation and microgravity on biological material

"Miniatura 6" DEC-2022/06/X/NZ1/01309 (2022-2023) - "Assessment of the biological properties of membranes made of poly-L-lactide supplemented with platelet-rich fibrin" (leader: Agnieszka Chwiłkowska, PhD)

Opus 21 2021/41/B/ST5/02233 (2022-2026) - "Structure and function of the protein crown on the surface of nanoparticles", a project in cooperation with Jerzy Haber Institute of Catalysis and Surface Chemistry of the Polish Academy of Sciences (leader: Prof. B. Jachimska, leader of the part of Wroclaw Medical University - J. Kulbacka) https://projekty.ncn.gov.pl/opisy/519012-pl.pdf

Daina 2 UMO-2020/38/L/NZ7/00342 (2021-2024) - "Manipulation of drug resistance in cancer cells through nanosecond asymmetric pulse sequences" - (J. Kulbacka) NCN info

Sonata Bis 6 2016/22/E/NZ5/00671 (2017-2022) - "Involvement of ultra-short electrical pulses in the induction of oxidative stress and assessment of the anticancer potential of nanosecond electroporation (nsPEF) in colorectal cancer in an in vitro and in vivo model" - (J. Kulbacka) National Science Center_info

Preludium 14 (2018 - 2021) - "Sensitisation of pancreatic cancer cells to pulsed electric fields and electrochemotherapy - from molecular modelling to miniaturised 3D cell cultures" - (O. Michel) National Science Center info

Preludium 12 (2017-2020) - "Effects of calcium ions and calcium ions with electroporation on intracellular mechanisms of normal and pathological skeletal muscle cells of rats" - (A. Szewczyk) National Science Center_info

Preludium 10 (2016-2019) - "Assessment of the effects of electroporation and electrochemotherapy on human cancer cells sensitive and resistant to chemotherapy in vitro" - (N. Rembiałkowska) National Science Center_info

The Ministry of Science and Higher Education projects:

Student Scientific Associations Create Innovations (2022- 2023) - "Nutraceuticals in the fight against the carcinogenic effects of smog"- Student Scientific Association of Cancer Cell Biology (supervisor: J. Kulbacka, student leaders: Z. Łapińska and U. Szwedowicz)

Student Scientific Associations Create Innovations (2021- 2022) - "Electrical impulses as a tool to modulate the CD8+ lymphocyte immunophenotype" - Student Scientific Association of Cancer Cell Biology (supervisor: J. Saczko, student leader: W. Szlasa)

Diamond Grant 8th edition - "Sonoporation as a modern method of modulation of gastrointestinal cancer cell membranes in anticancer therapy" (2019-2023) (student leader: D. Przystupski, supervisor: J.Kulbacka)

Best of the Best 3.0! (POWER) - "Research on the preservation of biological material for space missions" (supervisors: J.Kulbacka - Wroclaw Medical University; J. Górski - PWr) (2018-2019) The Ministry of Science and Higher Education_info 

Best of the Best  4.0 (POWER) - "Modern anticancer therapies - the effects of magnetic field and nanosecond electrical impulses on modulation of melanoma immunophenotype" (supervisors: J.Kulbacka, K. Karłowicz-Bodalska) (2022-2023)

The National Centre for Research and Development projects

POiR "Lower Silesian Biomedical Technology Zone" - Bio-nanosatellite using miniaturised lab-on-chip instruments and the methodology for bio-medical research using it in microgravity conditions (SatRevolution, PWr, UMED, IITD, UP).info

Young Scientists projects

STM.A040.18.013 "Effects of modulators of retinoid receptor expression and natural substances penetrating the blood-brain barrier on human neoplastic and normal nerve cells." (A.Choromańska)

STM.2020 "Effect of atorvastatin on the effectiveness of electrochemotherapy with calcium - in vitro studies with the use of steroidogenic cells" - (N. Rembiałkowska)

Regional Initiative of Excellence projects; Strategic Area no. 2

RN-N/1798-420/2020 - "Molecular alterations induced by simulated microgravity and their implications to drug resistance of human cancer cells" (J. Kulbacka)
RN-N/294-420/2020 - "Natural substances in neutralisation of simulated muscle atrophy under in vitro conditions” (M. Wastag, J. Kulbacka)

Year 2021 - pdf

Year 2020 - pdf

Year 2019 - pdf

Year 2018 - pdf

Student Scientific Association of Cancer Cell Biology -KoKoN

 K148, FB

Supervisors:
Anna Choromańska, PhD
Nina Rembiałkowska, PhD, MSc

Chair
Magdalena Kloc
magdalena.kloc@student.umw.edu.pl

Deputy Chair

Elżbieta Bartoszewska
elzbieta.bartoszewska@student.umed.wroc.pl

Office assistant

Klaudia Molik
klaudia.molik@student.umed.wroc.pl

Planned scientific events:

https://konferencje.umw.edu.pl/5iwsm2023/

Research filed:

Currently, cancer is one of the main causes of death. To meet the problems of today's world, we are engaged in research in the field of new therapies (e.g. chemotherapy (CT), electrochemotherapy (ECT), photodynamic therapy (PDT)) that can be used in oncology in the future. Through specialised molecular research, we are deepening and developing our interests in the field of cancer cell biology. The subject of our activity is the preparation, conduct and promotion of research projects on topics related to cancer cells. 

Research projects underway:

1. The influence of cyanine dyes in photodynamic therapy on the autophagy process and the influence of chloroquine on the effectiveness of therapy on the melanoma cell line (C32) in an in vitro study - Natalia Lange

2. The effect of an alternating magnetic field and dexamethasone on telomerase in leukemia cells - Ela Bartoszewska, Klaudia Molik, Magda Kloc, Monika Kisielewska

3. Discovery of anti-cancer compounds from Wrocław's nature: endophytes from the Mokrzanski Forest - Magda Kloc

Achievements:

1. 1st place in the national competition for scientific associations "3Mind" organised by 3M

2. Winners of the "Best of the best! 3.0" competition organised by the Ministry of Science and Higher Education for the project: "Research on the preservation of biological material for space missions"

3. Research at the European Space Agency (ESA) info

4. Winners of the "STRUNA" competition in the StRuNa-Med 2019 category and in the main category Project of the Year 2019

5. Winners of the StruNa competition in the StruNa-Med 2020 category.

6. Winners of the Pro Juvenes 2022 award in the "Scientific Project" category (the plebiscite is organized by the Students' Parliament of the Republic of Poland)

7. StRuNa-Med 2023 - distinction in the Students' Science Club of the Year 2023

8. Title of Winner in the Talents of Jutra 2023 program, implementation of the project "Hydrogels with nanoparticles of anticancer compounds produced by endophytic fungi as a new form of skin cancer therapy" (Magda Kloc)

Recruitment for MA theses at the Faculty of Pharmacy at Wroclaw Medical University 2023/2024 based on the rules under the link DIPLOMA THESES

Topics of MA theses in the Pharmacy and Analytics major 2022/23

1. Effects of resveratrol on estrogen receptor expression in cancer cells.
2. Effects of curcumin on estrogen receptor expression in cancer cells.
3. Effect of nanosecond electrical impulses on the expression of LAG-3 and PD-1 surface markers on pigmented (A375) and non-pigmented (C32) melanoma cells - (implementation under FAST WCA - reserved)
4. Effect of electroporation on the expression of protein markers of apoptosis in lung cancer cells.
5. Effect of electroporation on the expression of drug resistance proteins in lung cancer cells.
6. Effects of oncosomes on human normal cells.
7. Assessment of the biocompatibility of poly(glycerol adipate) composite biomaterials with the addition of bioglass with human fibroblasts in vitro.
8. Analysis of c.818 G>A mutation frequency in the TP53 gene in children with cataract (Analytics)